摘要
目的: 探讨实验动物和患者接受不同方法处理的同种异体皮质骨板移植后宿主免疫学反应。方法: 48只山羊接受分别经过 -70℃深低温冷冻 4周、 -70℃深低温冷冻 4周 +48℃环氧乙烷灭菌、-70℃深低温冷冻 4周+25kGyγ射线辐照处理的同种异体皮质骨板移植,对照组移植异体山羊新鲜皮质骨板。7例髋关节置换术后股骨假体周围骨折患者, 接受深低温冷冻和环氧乙烷处理同种异体皮质骨板移植重建股骨骨折。检测实验动物外周血CD4+、CD8+以及患者外周血T淋巴细胞亚群、抗体、补体和循环免疫复合物。结果: 新鲜骨板移植组CD4+明显升高, 术后 6周达到顶峰。除深低温组术后 6周CD4+升高外, 深低温冷冻、深低温冷冻 +环氧乙烷及深低温冷冻 +γ射线辐照组T淋巴细胞亚群无明显改变。患者T淋巴细胞亚群、抗体和免疫复合物正常, 切口无感染。结论: 新鲜同种异体皮质骨板具有抗原性, 移植后诱发宿主免疫排斥反应; 深低温冷冻、深冻+环氧乙烷和深冻 +γ射线辐照处理同种异体皮质骨板移植受体无明显免疫排斥反应。
Objective: To discuss the immune response of cortical bone plates allografts treated by deep freezing, ethylene oxide sterilization and gamma rays radiation in animal and patients.Method:Forty eight goats underwent allogeneic cortical bone plates transplantation treated by deep freezing, deep freezing plus 48℃ ethylene oxide, and deep freezing plus radiated with 25kGy gamma rays as well as fresh cortical bone plates. Groups of goats were sacrificed and specimens were procured at 3 ,6 ,12 ,and 24 week after surgery for examination CD4 +, CD8 + in blood.Seven patients with periprosthetic fractures of hip underwent cortical bone plates allografts by deep freezing at -70℃ after being treated of 48℃ ethylene oxide.To detect T lymphocytes,antibody,complement and circulation immunity complex (CIC) in blood.Result:Animal experiment showed that CD4 + in blood increased, and the step up of T lymphocytes achieved the peak at 6 week post operation in the fresh bone plates group.T lymphocytes of groups in blood were normal in deep freezing, deep freezing+ethylene oxide and deep freezing+radiation with 25kGy gamma rays groups in animal. T lymphocytes, antibody, and circulation immunity complex (CIC) in blood were normal post operation in patients.Conclusion:There is strong antigen and immune response in allogeneic fresh cortical bone plates transplantation, but there are no immune response in animal and patients who received allograft strut treated by deep freezing, deep freezing plus 48℃ ethylene oxide, and deep freezing plus radiated with 25kGy gamma rays.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2005年第3期201-203,共3页
Orthopedic Journal of China
关键词
移植
同种异体
骨板
免疫反应
Transplantation,homologous
Bone plates
Immune response
