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低分子肝素对肝癌生长和转移抑制作用的研究 被引量:11

Inhibition of low molecular weight heparin on the growth and metastasis of human hepatocarcinoma implanted in nude mice
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摘要 目的 研究低分子肝素对肝癌生长和转移的抑制作用。方法 建立人肝癌裸鼠动物模型 (LCI D2 0 )。术后第一天起分别予腹腔内注射生理盐水 (对照组 )、顺铂 +氟尿嘧啶 (化疗组 )、皮下注射法安明 (低分子肝素组 )、顺铂、氟尿嘧啶与法安明联合应用 (联合组 ) ,连续 5周。测量原位肿瘤大小、观察肿瘤腹壁、其他脏器转移及腹水情况 ;肿瘤组织中微血管密度 (microvesseldensity,MVD)、血管内皮生长因子 (vascularendothelialgrowthfactor,VEGF)、平滑肌肌动蛋白 (smoothmuscleactin ,SMA) ;测定血清AFP、CEA、CA199。结果 低分子肝素组肿瘤体积、MVD、SMA、肝、肺、腹壁转移率及腹水形成率明显低于对照组 (P <0 0 5 ) ,抑瘤率明显高于对照组 (P <0 0 5 )。结论 低分子肝素对肝癌的生长与转移具有抑制作用 ,该作用与抑制肿瘤血管形成有关。 Objective To study the effect of low molecular weight heparin on the growth and metastasis of hepatocarcinoma in vivo Methods Metastatic model simulating human hepatocarcinoma was established by orthotopic implantation of the tumor tissue into nude mice Animals were randomly divided and submitted to different treatment groups: control group treated with nornal saline, chemotherapy group with 5 FU and CDDP i p , Fragmin group (low molecular heparin), combination group After 5 weeks of treatment, tumor volume、 microvessel density (MVD)、 VEGF smoothmuscle actin (SMA) and metastasis in Fragmin group significantly decreased and tumor inhibitive rate increased ( P <0 05) Results Conclusion Low molecular weight heparin inhibits growth and metastasis of hepatocarcinoma, possibly by inhibiting tumor angiogenesis Combination of low molecular weight heparin with chemotherapy could be more effective
出处 《中华普通外科杂志》 CSCD 北大核心 2004年第12期758-760,共3页 Chinese Journal of General Surgery
基金 上海市卫生局资助项目 (0 0 44 1)
关键词 低分子肝素 肝癌 癌生长 对照组 抑制作用 转移 法安明 腹水 血管内皮生长因子 顺铂 Hepatocellular carcinoma Neoplasm metastasis Heparins Angiogenesis inhibitor
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参考文献5

  • 1Carmeliet P, Jain RK. Angiogenesis in cancer and other disease. Nature, 2000,407:249-257.
  • 2Collen A, Smorenburg SM, Peters E, et al. Unfractionated and low molecular weight heparin affect fibrin structure and angiogenesis in vitro. Cancer Res,2000,60:6196-6200.
  • 3El-Assal ON, Yamanoi A, Ono T, et al. The clinicopathological significance of heparanase and basic fibroblast growth factor expressions in hepatocellular carcinoma. Clin Cancer Res, 2001,7:1299-1305.
  • 4Ornstein DL, Zacharski LR. The use of heparin for treating human maliganancies. Haemostasis, 1999,29(suppl 1):48-60.
  • 5Drixler TA, Borel Rinkes IHM, Ritchie ED, et al. Continuous administration of angiostatin inhibits accelerated growth of colorectal liver metastases after partial hepatectomy. Cancer Res, 2000,60:1761-1765.

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