摘要
目的 :探讨我国陕西地区宫颈癌的发生与人类白细胞抗原 (HLA) DQB1 0 3、DR15等位基因的相关性 .方法 :采用序列特异性引物的聚合酶链式反应 (PCR SSP)方法 ,扩增HLA等位基因DQB1 0 3、DR15的目的DNA片段 (分别为 79、197bp) ,分析两对等位基因在陕西地区宫颈癌患者和正常人中分布频率的差异 .结果 :4 5名宫颈癌患者组中DQB1 0 3等位基因频率显著高于 5 0名健康对照组 ,DR15等位基因频率在两组中的分布无显著差异 .结论 :HLA等位基因DQB1 0 3可能与宫颈癌的发生具有相关性 ,DR15可能与宫颈癌的发生不存在关联 .
AIM: To investigate the relationship between the susceptibility to cervical cancer and HLA-DQB1*03 and DR15 alleles in Shaanxi Province. METHODS: The target DNA fragments of HLA-DQB1*03 and DR15 alleles (79 and 197bp respectively) were amplified by using polymerase chain reaction of sequence specific primers(PCR-SSP)in cervical cancer patients and healthy control in Shaanxi Province. RESULTS: The frequency of the DQB1*03 allele was significantly higher in cervical cancer patients than that in normal controls, while no significant difference was found in the frequency of the DR15 allele between the patient group and the control group. CONCLUSION: There may be some correlation between the development of cervical cancer and HLA-DQB1*03, and no correlation between the development of cervical cancer and HLA-DR15.
出处
《第四军医大学学报》
北大核心
2004年第24期2264-2266,共3页
Journal of the Fourth Military Medical University
