摘要
目的 研究P38信号通路 (P38mitogen activatedproteinkinase,P38MAPK)在大鼠肾小球系膜细胞 (ratmesangialcells,RMCs)表达血管内皮生长因子 (vascularendothelialgrowthfactor,VEGF)中的作用 ,从而探讨P38MAPK在糖尿病肾病中的作用机制。方法 分别以高葡萄糖、糖基化终末产物 (Advancedglycationendproduct ,AGEs)、高胰岛素和过氧化氢孵育RMCs;以P38MAPK特异性抑制剂SB2 0 35 80预处理RMCs,再以上述 4种因素孵育RMCs,观察RMCsP38MAPK和VEGF蛋白的表达。结果 4种刺激因素均可独立激活P38MAPK ,VEGF表达量也明显增加 ;SB2 0 35 80预处理后 ,VEGF表达被明显抑制。结论 P38MAPK是VEGF的上游激酶 ,P38MAPK和VEGF可能参与了糖尿病肾病的发生发展。
Objective To investigate the role of P38 mitogen-activated protein kinase (P38MAPK) for expression of VEGF in rat mesangial cells and to study the mechanism of P38MAPK on diabetic nephropathy. Methods We initially investigated protein expression of P38MAPK and VEGF in rat mesangial cells (RMCs) which were incubated respectively with 25 mM glucose, 100 mg/ml BSA-AGEs, 100 nM insulin and 100 mM H 2O 2. We also studied the relationship between P38MAPK and VEGF protein expression by using SB203580, a specific inhibitor of P38MAPK.Results P38MAPK and VEGF had significantly higher expression in rat mesangial cells incubated with 25 mM glucose, 100 mg/ml BSA-AGEs, 100 nM insulin and 100 mM H 2O 2 respectively. VEGF expression was significantly reduced when P38MAPK was inhibited by SB203580.Conclusion P38MAPK and VEGF are involved in development of diabetic nephropathy and P38MAPK stimulation is essential for VEGF expression.
出处
《重庆医学》
CAS
CSCD
2005年第1期16-18,共3页
Chongqing medicine
关键词
P38信号通路
VEGF
大鼠肾小球系膜细胞
糖尿病肾病
P38 mitogen-activated protein kinase
vascular endothelial growth factor
diabetic nephropathy
rat mesangial cells
