摘要
为了研究戊糖乳杆菌31-1菌株产生的细菌素的性质,采用SPSepharoseFastFlow阳离子交换树脂纯化细菌素,结果发现选用含0.1MNaCl的0.02M醋酸盐缓冲液即可洗脱下细菌素峰,比活力达到1259.84(AU/mg),是原发酵液的17倍,并且采用Tricine-SDS-PAGE法估测出细菌素的分子量大小在14.2kDa以下。
Bacteriocin produced by Lactobacillus pentosus 31-1 was purified by ammonium sulphate precipitation, followed by SP-Sepharose FF cation exchange chromatography. And the bacteriocin was washed with 0.1M NaCl (in acetate buffer, pH5.5), at 1.2ml/min. And the specific activity was 1259.84AU/mg. The molecular size of bacteriocin was estimated to be under 14.2 kDa, according to Tricine-SDS PAGE.
基金
国家863资助项目(2002AA248041)
科技部"十五攻关"项目(编号2001BA501A11)。
