摘要
目的:探讨树突状细胞(S 100)、抑癌基因p63、金属蛋白酶组织抑制因子 1(TIMP 1)在喉癌组织中 的表达及与吸烟、饮酒、病理学分级、临床TNM分期和预后等因素的关系。方法:应用组织芯片技术和免疫组织 化学SP法检测85例喉鳞状细胞癌组织中S 100、p63、TIMP 1等因子的表达。结果:85例中,6例组织芯片脱落 或无肿瘤细胞。对79例进行S 100检测,其中47例(59.5%)出现阳性细胞(0～34个/视野)。S 100阳性树突状 细胞浸润程度与喉癌分化程度、临床分期差异均有统计学意义(均P<0.05)。对79例进行TIMP 1检测,其中 35例(44.3%)为阴性表达,44例(55.7%)为阳性表达。TIMP 1蛋白阳性表达与患者肿瘤临床分期、有无淋巴结 转移及预后差异均有统计学意义(均P<0.05)。在79例中,58例p63表达阳性(73.4%),平均染色比率为 45.5%,但未发现与临床因素相关。结论:TIMP 1、S 100与肿瘤的生长、分化有关,可作为临床检测预后的重要 参考指标。
Objective:To evaluate the predictive role of S-100 positive dendritic cell, tissue inhibitor of metalloproteinases-1 (TIMP-1) and p63 gene in primary laryngeal carcinoma with epidemiology(smoking and drinking), histological grading, surgical treatment,TNM stage and prognosis by the tissuechip technology. Method:We studied the expression of dendritic cell(S-100), TIMP-1 and p63 gene on a series of 85 primary laryngeal carcinoma patients who had ever received in our hospital between 1992 and 2000 by the tissuechip technology and SP method.The correlation of each score according to the intensity and percentage of labeled cells or intercellular substance with relevant clinical dada was statistically analyzed. Result:Some cases were lost or boasted no tumor tissue in our tissuechip. In available 79 patients, the rate of expressing S-100 positive dendritic cell is 59.5% (47/79), and the average percentage of its labeled cells in them is 8.71%.S-100 positive dentritic cells showed significent difference among different pathological grade group,early and late stage(P<0.05). The rate is 55.7%(44/79) of the specimens whose basal membrane and extracellular matrix was strongly stained by TIMP-1;There was statistical significand in TIMP-1 protein demonstration between early and late stages, lymph node metastasis and 3-year survival rate (P<0.05) by chi-square test, but no relation with smoking, drinking, gender, age and histological classes (P>0.05).There was wo statistical significand in p63 protein demonstration between TNM stages, lymph node metastasis, 3-year survival rate, smoking, drinking, gender, age and histological classes (P>0.05). Conclusion:The tissue microarray technique spent shorter time and less expense, and showed higher consistency in our essays. And the present study suggests TIMP-1 and S-100 conld be the clinical discriminators in laryngeal carcinoma.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2005年第3期127-129,共3页
Journal of Clinical Otorhinolaryngology
基金
上海市科委基金资助项目(No:市4119181)
