摘要
目的 观察丝裂原活化蛋白激酶 (MAPKs)在外源性一氧化碳 (CO)抗大鼠肢体缺血再灌注 (IR)所致肺损伤中的作用。方法 健康SD大鼠 ,随机分为 4组 (每组n =8) :对照组 (Con trol)、Control +CO、IR和IR +CO组。复制大鼠双后肢缺血及再灌注后肺损伤模型。IR +CO和Control +CO组在再灌注前 1h或相应时间点置含CO的空气中 ,其余两组呼吸空气。观察大鼠肺组织学、肺组织中中性粒细胞 (PMN )数目、肺组织湿重和干重之比 (W /D)、丙二醛 (MDA)含量以及动物生存情况变化。应用Westernblotting检测肺组织中三种磷化MAPKs ,即细胞外信号调节激酶(ERK)、c Jun氨基末端激酶 (JNK)和 p3 8表达的变化。 结果 与Contorl组相比 ,IR组动物死亡率、肺组织PMN数目、W /D、MDA含量以及磷酸化ERK、JNK和p3 8表达均显著增高 ;与IR组相比 ,IR +CO组IR组动物死亡率、肺组织中PMN数目、W /D和MDA含量均显著降低、肺损伤减轻 ,p3 8表达显著增高 ,JNK表达显著降低 ,ERK表达无显著变化。结论 MAPKs信号通路参与了外源性CO抗大鼠肢体IR所致肺损伤作用的分子机制。
Objective To observe the role of mitogen-activated protein kinases (MAPKs) in protection of exogenous carbon monoxide (CO) against lung injury induced by ischemia-reperfusion (IR) of hind limb in rats.Methods Healthy SD rats were randomly divided into 4 groups:control,control+CO,IR and IR+CO.An animal model of ischemia in hind limbs and the reperfusion lung injury was made.The rats in IR+CO and control+CO groups were exposed to air containing CO at 1 h before reperfusion or thecorresponding control time point,while the other two groups were exposed to theroutine air.The lung tissue structure,polymorphonuclear leukocyte (PMN) count,wet-to-dry weight ratio (W/D),malondialdehyde (MDA) content and the animal survival rate were assayed.The phosporylated forms of extracellular-signal regulatedprotein kinase (ERK),c-Jun NH2-terminal kinase (JNK) and p38 MAPKs in the lung were detected by Western blotting.Results Compared with the control group,the animal death rate,lung PMNs number,W/D,MDA content and the activated protein levels of ERK,JNK and p38 were all significantly increased in IR group.As compared with the IR group,the animal death rate,lung PMNs number,W/D,MDA content and the p-JNK protein level were all significantly decreased,while the p-p38 protein level was increased in IR+CO group.There was no significant difference in the p-ERK expression between the IR and IR+CO groups.Conclusion MAPKs pathway might be involved in the protective mechanims of CO against the lung injury induced by limb IR in rats.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第2期163-165,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目 (30 2 71 337)
