摘要
目的探讨p63基因和p73基因蛋白与毛细血管瘤发生发展的关系。方法采用免疫组化SP法检测40例儿童毛细血管瘤和20例正常人皮肤组织p63基因和p73基因蛋白的表达;对所获得的检测结果进行图像分析处理。结果在正常人皮肤组、增生期与退化期血管瘤中,p63和p73蛋白的平均吸光度分别为0.923±0.191,0.953±0.120;8.271±1.953,6.408±2.151;0.920±0.187,1.073±0.516;阳性面积率分别为0.106±0.014,0.087±0.012;0.370±0.071,0.184±0.015;0.116±0.012,0.098±0.014。增生期组与退化期组、正常人皮肤组分别比较,p63和p73阳性表达的差异均有统计学意义(P<0.05),退化期组与正常人皮肤组之间,p63阳性表达的差异无统计学意义(P>0.05)。结论p63基因在毛细血管瘤中并未作为肿瘤抑制基因起作用,相反是作为癌基因而促进内皮细胞的增殖,可能与血管形成关系密切。p73在毛细血管瘤的增生中起着重要作用。
Objective To investigate the relationship between the expression of p63, p73 proteins and the development of hemangioma. Methods The immunohistochemical technique and quantitative image analysis were used to detect the expression of p63 and p73 proteins in 40 cases of capillary hemangioma and 20 specimens of normal skin. Results The absorbance value (mean ± SD) of p63 and p73 expression in normal skin tissue, proliferative phase of hemangioma and involuting phase of hemangioma were 0.923 ± 0.191 and 0.953 ± 0.120, 8.271 ± 1.953 and 6.408 ± 2.151, 0.920 ± 0.187 and 1.073 ± 0.516, respectively. The expression of p63 and p73 in proliferative phase of hemangioma was significantly increased as compared with those in involuting phase of hemangioma and normal skin tissue (P < 0.05), while no significant difference of p63 and p73 expression was observed between involuting phase of hemangioma and normal skin tissue (P > 0.05). Conclusions It is suggested that p63 gene is not a tumor suppressor gene but an oncogene in hemangioma and may contribute to the proliferation of endothelial cell and be associated with angiogenesis, and p73 may play an important role in the proliferation of hemangioma.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2005年第2期89-91,共3页
Chinese Journal of Dermatology
基金
国家自然科学基金资助项目(30271345)
湖北省科技攻关资助资助项目(301130656)
