摘要
本工作采用腹腔注射5-羟色胺合成抑制剂对氯苯丙氨酸降低脑内5-HT含量的方法,观察了脑内5-HT系统在达乌尔黄鼠自然冬眠和脑室注射6-羟多巴胺促进入眠效应中的作用。结果表明;在自然情况下,达乌尔黄鼠入眠后脑内5-HT含量增加,腹腔注射对氯苯丙氨酸后动物入眠诱导期明显延长;在脑室注射6-羟多巴胺人为地降低脑内NE系统活动的条件下,脑内5-HT含量即使处于较低水平,动物也能很快入眠,但入眠时脑内5-HT含量需要恢复到一定的基础水平。提示脑内NE系统功能活动的降低或/和5-HT系统功能活动加强同是触发动物入眠的重要因素。周期性入眠和觉醒可能依赖于脑内这两个系统功能活动的平衡。
In this work the role of brain serotonergic system in natural hibernation and hibernation induced by i.c.v. injection of 6-hydroxydopamine were studied in ground squirrel (Citellus dauricus) after intraperitonal administration of para-chlorophenylalanine (pACA) , a specificdepletor of brain serotonin. The results showed: ( 1 ) The content of 5-HT in whole brain, after pCPA injection was depleted to 42% of control value at 2nd day, and reached to the lowest at 4th day, about 19% of control, then gradually recovered with time, arrived at 84% of control value at 16th day. ( 2 ) In the natural hibernating ground squirrels, the induction period for hibernation were 26±6 days, and 5-HT content in brain increased when compared with the euthermic animals, while the induction period for hibernation in ground squirrels pretreated with pCPA were 35±5 days, significantly longer than that of natural hibernating animals. (3) The ground squirrels treated with i.p. pCPA+i.c.v.6-OHDA were able to enter into hibernation when 5-HT content in brain has not recovered to the control level, however the induction period for hibernation (15±5 days) was longer than that of animals treated with i.p. saline +i.c.v.6-OHDA, indicating that a basal 5-HT level was still necessary for hibernating under the condition of i.c.v. injection of 6-OHDA.
These results suggested that both the decrease of NE system activity or/and the increase of 5-HT system in brain are the important controlling factors in triggering the onset of hibernation.
出处
《兽类学报》
CAS
CSCD
北大核心
1993年第3期211-216,共6页
Acta Theriologica Sinica
基金
国家自然科学基金资助项目
关键词
达乌尔黄鼠
冬眠
氯苯丙氨酸
Ground squirrel (Citellus dauricus)
Hibernation
Para-Chlorophenylalanine
Serotonin
Norepinephrine
