摘要
目的:研究甲状腺机能减退大鼠海马组织T3 核受体mRNA的变化规律及半硫丸对其的影响,探讨半硫丸对甲减的作用机理。方法:Wistar大鼠随机分为正常对照组和造模组,造模大鼠每日腹腔注射PTU诱发甲状腺机能减退动物模型后,分为模型组、甲状腺素片组、半硫丸组,治疗组分别予相应药物治疗 30d。采用RT PCR法测定大鼠脑海马组织T3NRα1mRNA和T3NRβ1mRNA的表达水平。结果:甲减大鼠海马组织T3NRα1mRNA和T3NRβ1mRNA的表达水平明显下降,与正常组比较有显著性差异 (P<0.01)。两种药物均可明显增强甲减大鼠海马组织T3NRα1mRNA和T3NRβ1mRNA的表达,与模型组比较均具有显著性差异,治疗组之间无明显差异。结论:半硫丸可通过上调甲减大鼠海马T3核受体mRNA表达水平,增加T3核受体数目,改善甲减造成的脑神经损伤。
To observe the change of T 3NR mRNA in hippocampus of the rats of hypothyroidism and the effect of “Banliu Bonu” on it in order to study its protective mechanism on brain injury due to hypothyroidism, the rats were randomly divided into normal control group and hypothyroidism group. Then the rats in hypothyroidism group were further divided into model group, thyroxin group and “Banliu Bonu” group after they were intraperitoneally injected with PTU to evoke hypothyroidism. Corresponding drug treatments were applied for the treatment groups respectively. The expressions of T 3NRα1 mRNA and T 3NRβ1 mRNA in hippocampus of the rats were detected with RT-PCR. Results: The expressions T 3NRα1 mRNA and T 3NRβ1 mRNA decreased obviously, with significant differences compared with normal group (P<0.01); while their expressions in treatment groups increased, with a great difference as compared with model control group (P<0.01), and there was no difference between treatment groups (P>0.05). Conclusions: “Banliu Bonu” may remedy the injury of cranial nerve due to hypothyroidism by up-regulating the expression of T 3NR mRNA and increasing nuclear T 3 receptor to nourish nerves and facilitate the recovery of neuronal function.
出处
《上海中医药杂志》
北大核心
2005年第2期46-48,共3页
Shanghai Journal of Traditional Chinese Medicine
