摘要
采用离体灌流孵育和腹腔注射方法,研究鲑鱼促性腺激素释放激素(GnRH)类似物 sGnRH-A(Arg^6Trp~?Leu^3Pro~?NEt-LHRH)和哺乳类GnRH类似物LHRH-A(Ala^5Pro~?-NEt-LHRH)对鲤鱼脑垂体生长激素(GH)分泌的直接刺激作用以及对草鱼鱼种 GH 分流和生长的促进作用。2分钟脉冲式 sGnRH-A 和 LHRH-A 以剂量依存形式直接刺激离体灌流孵育的鲤鱼脑垂体 GH分泌,且 sGnRH-A 刺激GH 分泌活性显著高于 LHRH-A。腹腔注射两种剂量(0.01μg/g 体重/周和0.001μg/g 体重/周)的 sGnRH-A 或LHRH-A(每周一次,共 6周)都能显著提高草鱼鱼种相对体重和相对体长生长率,并促进草鱼鱼种血清 GH 水平提高。sGnRH-A 和 LHRH-A 促进生长的作用是剂量依存的,且sGnRH-A的促进生长作用显著高于 LHRH-A。
The directly stimulating actions of salmon GnRH analog sGnRH-A (Arg^6TrP^7Lou^8Pro^9NEt--LHRH) and mammalian GnRH analog LHRH-A(Ala^6Pro^9NEt--LHRH) on growth hormone (GH) secretion from pituitary of com-mon carp (Cyprinus carpio L.), and the effects of LHRH-A and sGnRH-A on se-rum GH levels and growth of grass carp fingerling were studied by using an invitro perifusion system and in vivo treatment respectively. Two--min pulses ofsGnRH--A or LHRH-A (1, 10 and 100nM), in dose-dependent manner, directlystimulated GH secretion from perifused pituitary fragments of common carp. Thein vitro GH-releasing potency of each dose of sGnRH--A was significantly higherthan that of the same dose of LHRH--A. Intraperitoneal injection of sGnRH--A or LHRH--A(0. 01μg/g,b.w./week and 0. 001μg/g, b.w./week, once a week, total of sixweeks) resulted in significant increase in relative somatic growth rate (RSGR)and relative linear growth rate (RLGR) and in increase in serum GH levels ofgrass carp fingerling. The inductions of LHRH-A and sGnRH--A on growth ofgrass carp fingerling were dose--dependent, and the increases in RSCR andRLGR induced by sGnRH-A were significantly higher than those induced byLHRH-A. These results indicated that GnRH analog can directly stimulate GHsecretion from pituitary and induce increase in growth rate of cultured fish. Therelationships between structure and function of GnRH analog in inducing GHsecretion and grewth are similar to those in stimulating gonadotropin secretionin teleost.
出处
《水产学报》
CAS
CSCD
北大核心
1993年第4期282-288,共7页
Journal of Fisheries of China
关键词
促性腺激素
鱼类
生长激素
分泌
GnRH
growth hormone
growth
common carp
grass carp
