摘要
选择6株自堆腐处理2阶段的石油污染土壤中分离的真菌,另引进2株白腐真菌作为供试菌株,采用以河沙替代土壤,用人工投加污染物的实验方法,研究了有较强解脂酶活性的真菌对特稠油、胶质和沥青质的降解效果。结果表明,在好氧条件下,土壤中低浓度污染物(特稠油浓度3.76g·kg-1、胶质和沥青质浓度0.095g·kg-1)经过80d的堆腐处理后,黄孢原毛平革菌(PhanerochaeteChrysoporium)、采绒革盖菌(Cviolusversicolor)、木霉(Trichodermasp.)、小克银汉(Cunninghamellasp.)、曲霉(Aspergillussp.)、镰刀菌(Fusariumsp.)对特稠油有较好的降解效果,降解率分别为20.40%、19.14%、22.46%、15.88%、16.48%和14.95%。但仅采绒革盖菌和小克银汉对胶质+沥青质有一定的去除效果,其降解率分别达到11.47%和11.58%。本研究筛选的两菌株为强化难降解石油组分的去除提供了可能。
In the process of bio-remediation of soil contaminated with petroleum hydrocarbons, the degrading rate would continuously drop along with remedying time. The remediation of persistent petroleum hydrocarbons has been a key problem of soil remediation. Six strains of fungi separating from the soil contaminated with petroleum hydrocarbons in stage two of remediation process and the strains with potential abilities for degradation of persistent petroleum hydrocarbons were screened through analyses of lipase activities. Special thick oil, containing high concentration of resin and asphalt, was mixed with fine sand, with the concentration of oil and resin with asphalt being 3.76 g·kg-1 and 0.095 g·kg-1, respectively. The prepared bed was operated under aerobic condition.The results indicated that 6 strains of fungus showed higher degrading rate forspecial thick oil and resin with asphalt during the treatment period of 80 days. The degradation rates of Phanerochaete Chrysoporium, Cviolus versicolor, Trichoderma sp., Cunninghamella sp., Aspergillus sp., and Fusarium sp. for heavy thick oil were 20.40%, 19.14%, 22.46%, 15.88%, 16.48%and 14.95%, respectively. However, only Cviolus versicolo and Cunninghamella sp. had obvious degrading rates for resin and asphalt, which reached 11.47 %and 11.58 %respectively. These two strains of fungi may provide a possible channel for the degradation of persistent petroleum hydrocarbons in soils.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2005年第1期161-164,共4页
Journal of Agro-Environment Science
基金
国家高新技术研究发展计划项目(2002AA601210)
中国科学院沈阳应用生态研究所领域前沿项目(SLYQY0410)
国家自然科学基金项目(20277040
20337010)
关键词
生物修复
真菌
特稠油
胶质和沥青质
bioremediation
fungi
special thick oil
resin and asphalt
