摘要
将 GFP 基因标记的甲基对硫磷降解菌 DLLBR 接入 100 ml LB 培养基,过夜培养达到 1010cfu/ml,浇灌到盆钵试验的 800 g 土壤中,20 天后用激光共聚焦显微镜检测其在小青菜植株根部和植株内的定殖及分布。结果表明标记菌株能够在植株根圈较好地定殖,也能在植株内定殖。30 天后将植株各段研磨破碎,涂布 LB 平板计数发光菌落数,在根内的定殖数为 104cfu/g 根,在茎内的定殖数为 102cfu/g 茎。结果还发现与不接菌的对照相比,处理促进了植株内细菌群落结构的变化,尤其是芽孢杆菌的种类和数量明显增加。接菌 45 天后通过土壤计数检测发现,标记菌株在土壤中的存活力很高,能达到 106cfu/g 土,同时也发现与对照相比,接菌的土壤细菌群落结构明显发生了变化,细菌种类变化不大,但芽孢杆菌的数量明显增加。
Green fluorescent protein gene(gfp) labeled Methylparathion-Degrading Bacteria Pseudomonas putida DLLBR was cultured in LB medium overnight and then inoculated into the soil in pots with vegetable. After 20 days colonization and distribution of gfp-labeled DLLBR at the surface of the vegetable roots and inside the vegetable were visualized in a living state under a confocal laser scanning microscope. Microscopic observation show that the labeled bacteria had colonized well in the rhizosphere and inside the vegetable. After 30 days the population of DLLBR was counted through spreading the trituration of the sterilized vegetable to LB plates and found to have reached to 104cfu/g root and 102cfu/g stem. Results also show the introduced bacteria brought about some changes in the microbial communities inside the vegetable, especially Bacillus that increased in species and count. After 45 days, the DLLBR population in soil was 106cfu/g soil. In addition, we also discovered obvious changes in the microbial communities in the inoculated soil, but only in counts, not in species.
出处
《土壤》
CAS
CSCD
北大核心
2005年第1期100-104,共5页
Soils
基金
中国科学院南京土壤研究所开放室基金项目(2025102)
中国科学院知识创新项目( KZCX3-SW-417)资助。
关键词
GFP基因标记
激光共聚焦显微镜
研磨:植株内细菌群落结构
土壤细菌群落结构
Labeled by green fluorescent protein gene, Confocal laser scanning microscope, Trituration, The microbial communities inside the vegetable, The microbial communities in inoculated soil
