摘要
目的 比较免疫组织化学法与酶联免疫吸附法 (ELISA)测定胰岛细胞抗体 (ICA)的阳性率。 方法 对同一批 2 0 0份血清标本 ,在北京、上海两地三个三甲医院内分泌实验室分别采用免疫组织化学技术POPA法、ABC法及ELISA法检测ICA。 结果 免疫组织化学POPA法与ABC法检测ICA的阳性率和阴性率基本一致 ,而用ELISA法检测的阳性率低 ,假阳性率、假阴性率高。 结论 与ELISA法相比 ,用免疫组织化学法测定ICA的准确性高 ,重复性好 ,宜推广应用于临床。
Objective To compare the positive rate of islet cell antibodies(ICA) between immunohistochemical and ELISA assays Methods The ICA assays were performed of 200 serum samples in the three different laboratories by three methods of two immunohistochemical assays with peroxidase labeled protein A (POPA) and avidin biotin complex (ABC), and ELISA method Results The positive and negative rates of ICA by POPA and ABC methods were consistent, but was significantly lower by ELISA than by POPA and ABC methods Conclusions The present results demonstrate that ICA assays with immunohistochemical POPA and ABC techniques are more veracious and reliable than with ELISA, suggesting POPA and ABC could be widely used in clinical practice
