摘要
以非洲菊盆栽品种为材料,研究了BA和NAA不同浓度组合对不定芽诱导增殖的影响;基因枪介导法转化的不同轰击距离对转化率影响。结果表明:1.5 mg/L BA的培养基上增殖倍数最高,高达8.96倍;9 cm的目标轰击距离转化效率最高,达到10.9%。建立了非洲菊盆栽品种的再生和转基因体系,为非洲菊基因工程育种打下基础。
The in vitro regeneration and transformation of potted Gerbera jamesonii Bolus were investigated. The effects of hormone and hormone combination on the adventitious buds proliferation and target distance on the transformation frequency were studied. The results indicated that 1.5 mg/L BA significantly increased adventitious buds proliferation to 8.96-fold than that of hormone free treatment. 9 cm target distance significantly improved the transformation frequency, which was up to 10.9%.
出处
《细胞生物学杂志》
CSCD
北大核心
2004年第6期645-648,共4页
Chinese Journal of Cell Biology
关键词
盆栽
非洲菊
基因枪介导法
基因转化体系
培养基
再生体系
potted Gerbera jamesonii Bolus
regeneration system
particle bombardment
transformation system
transgenic plants
