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实验动物弓形虫感染的PCR法检测 被引量:2

The Detection of TOX Infection in Laboratory Animals by Using PCR
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摘要 通过自行设计引物,建立了一种检测实验动物弓形虫的PCR 方法,该方法具有高度特异性和敏感性。对几种实验动物的检测表明,购自农村的教学用兔体内可能有弓形虫,应予以重视。 A polymerase chain reaction (PCR) having high specificity and sensitivity was estibilished for detecting toxoplasma gondii (TOX) in laboratory animals, according to designing primers. The results showed that TOX might exist in rabbits (3/60) useful for teaching, which came from countryside. These findings suggest that the infection of TOX might be paid more attention in the aspect of laboratory animals. Key words TOX PCR mathematical statistics that the probability distribution of measured data obeys normal distribution. By the great difference in elastic moduli between cementum and dentin it is explained that 1 why more cementum grows at the root apexes;2 the behavior of cementum must be taken into consideration when the stress analysis for teeth is carried out.
出处 《中国实验动物学杂志》 1999年第3期168-170,共3页 Chinese Journal of Laboratory Animal Science
关键词 弓形虫感染 实验动物 PCR法 体内 特异性 敏感性 PCR方法 引物 检测实验 高度 human cementum elastic modulus normal distribution
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  • 1杨春贵,张丰雪,李亚鲁.弓形体、解脲支原体感染与男性不育的关系[J].泰山医学院学报,2003,24(4):387-389. 被引量:4
  • 2丛斌,姚玉霞,尤红煜,彭郁葱.聚合酶链反应检测实验动物弓形虫核酸的研究[J].中国实验动物学报,1998,6(2):9-12. 被引量:3
  • 3谷子林.近年来家兔主要传染性疾病——弓形虫病和附红细胞体病[J].中国农村科技,2005(4):39-41. 被引量:1
  • 4BURG J,ROVER C,POULETTY P.Direct and sensitive detection of apathogenic protozoan,Toxoplasma gondii,by polymerase Chain Reaction[J].Clin Microbiology,1989,27:1787-1792.
  • 5HAFLD J,GUICHARD D.Detection of Toxoplasma gondii by polymeraseChain Reaction in sera of Acutely[J].Parasitol,2000,86(4):857-859.
  • 6ELLIS J T.Polymerase Chain Reaction approaches for the detection ofNeospora caninum and Toxoplasma gondii[J].International J for Parasi-tol,1998,28:1053-1060.
  • 7HOMAN W L,VERCAMMEN M.Identification of a 200 to 300-fold repeti-tive 529bp DNA fragment in Toxoplasma gondii and its use for diagnosticand quantitative PCR[J].International J for Parasitol,2000,30:69-75.
  • 8SAVVA D.Isolation of a potential DNA probe for Toxoplasma gondii[J].Microbios,1989,58:162-172.
  • 9KHALIFA K,ROTH E A,ARASTEH K,et al.Value of PCR for evaluatingoccurrence of parasitemia in immunocompromised patients with cerebraland extracerebral toxoplasmosis[J].Journal of Clinical Microbiology,1994,32:2813-2819.
  • 10OWEN M R,TREES A J.Vertical transmission of Toxoplasma gondii fromchronically infected house and field mice determined by Polymerase ChainReaction[J].Parasitology,1998,116:299-304.

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