摘要
目的:研究氯化镁对大鼠缺血再灌注损伤心肌是否具有保护性作用,同时探讨氯化镁的心肌保护作用机制。方法:实验于2004-06/07在锦州医学院药理学实验室完成,选用50只SD大鼠,采用整体大鼠急性心肌缺血再灌注损伤模型,用3个不同剂量的氯化镁给大鼠尾静脉注射,测定心肌组织中Ca2+和丙二醛含量以及血清中乳酸脱氢酶含量,并同时记录心电图监测心肌缺血再灌注时心律失常的发生情况率。结果:氯化镁可降低血清中乳酸脱氢酶含量,也能降低心肌组织中的丙二醛和Ca2+含量。氯化镁可减少心肌缺血再灌注损伤时室性心律失常发生率和缩短心律失常的持续时间。给药组再灌注时ST段抬高(mV)程度(其中氯化镁低、中、高剂量组分别为0.16±0.03,0.12±0.02,0.06±0.01)较缺血再灌注组(0.22±0.06)显著降低。结论:氯化镁对心肌再灌注损伤具有保护作用,其机制可能与缓解心肌细胞内钙超负荷和抑制脂质过氧化有关。
AIM:To study whether magnesium chloride plays a protective role in myocardium of rats with ischemia reperfusion injury,and investigate the cardioprotective action mechanism of magnesium chloride. METHODS:The experiment was done in the Pharmacology Laboratory of Jinzhou Medical College from June through July 2004.Occlusion of anterior descending branch of left coronary artery(LAD) was performed in 50 SD rats to establish myocardial ischemia reperfusion injury models.Magnesium chloride at three difference dosages was injected via the tail vein. Malondialdehyde(MDA) and Ca2+Contents in myocardium, serum lactate dehydrogenase(LDH) and magnesium were assayed.ECG was recorded to monitor the incidence of cardiac arrhythmia after myocardial ischemia reperfusion injury. RESULTS:Administration of magnesium chloride was effective in reducing the incidence of arrhythmias,shortening the duration of arrhythmias,and decreasing the contents of MDA and Ca2+in the myocardium and the serum level of LDH.The elevation of the ST-segment of the ECG was decreased significantly in the low, middle and high dosage groups of magnesium chloride(0.16±0.03,0.12±0.02,0.06±0.01),as compared with the ischemia reperfusion group(0.22±0.06). CONCLUSION:Magnesium chloride has protective effects on myocardial ischemia reperfusion injury,and its mechanism may be related to relieving the calcium influx and reducing the products of lipid peroxidation in the myocardium.
出处
《中国临床康复》
CSCD
北大核心
2005年第3期78-79,共2页
Chinese Journal of Clinical Rehabilitation
