摘要
背景:电磁辐射在应用到许多领域的同时,对人体健康也构成一定的威胁。男性生殖系统也是电磁辐射的敏感靶器官,睾酮在电磁辐射条件下的变化及其机制目前还不清楚。目的:探讨电磁辐射对成年大鼠睾丸组织中类固醇合成急性调节蛋白(StAR)、细胞色素P450胆固醇侧链裂解酶(P450scc)的作用,初步揭示电磁辐射影响睾酮合成的分子机制。设计:随机对照研究。单位:解放军第三军医大学大坪医院野战外科研究所核医学科和劳动卫生学教研室。材料:本研究于2003-06/2004-05在解放军第三军医大学大坪医院野战外科研究所核医学科和劳动卫生学教研室完成。选择二级清洁级雄性成年Wistar大鼠75只,随机分为5组,即对照组以及电磁辐射后3,6,24,72h组,每组15只,体质量180～220g。方法:采用峰值功率为90W/cm2的微波连续辐照15min,辐照完毕后,动物均存活。采用放射免疫方法(RIA)分别测定电磁辐照后3,6,24,72h及对照组血清睾酮含量,同时运用RT-PCR方法测定睾丸组织中StAR、P450sccmRNA水平。主要观察指标:电磁辐照后3,6,24,72h组与对照组血清睾酮含量和StAR,P450sccmRNA的水平。结果:辐照后3h血清睾酮含量和StAR、P450sccmRNA水平均明显低于对照组,分别较对照组降低83.1%,57.3%,53.6%(P<0.01);6h略有回升,但仍明显低于对照组,分别较?
BACKGROUND:With the development of technology,electromagnetic irradiation has been applied in many fields;meanwhile,health of people could be threatened by electromagnetic exposure.Reproductive system of males is the major target of electromagnetic irradiation.But under the condition of electromagnetic exposure,the change of testosterone and its mechanism remain unknown. OBJECTIVE:To study the effect of electromagnetic irradiation on steroidogenic acute regulatory protein(StAR) and cytochrome P450 cholesterol side-chain cleavage(P450scc) in the tissues of the testis of the adult rats,and to preliminarily reveal the molecular mechanism of electromagnetic radiation that affects the synthesis of testosterone. DESIGN:A randomized controlled study. SETTING:Department of Nuclear Medicine and Staff Room of Labour Hygiene, Institute of Field Surgery,Daping Hospital of the Third Military Medical University of Chinese PLA. PARTICIPANTS:The study was completed in the Department of Nuclear Medicine and Staff Room of Labour Hygiene, Institute of Field Surgery,Daping Hospital of the Third Military Medical University of Chinese PLA from June 2003 to May 2004.A total of 75 male Wistar rats of clean degree II,weighing 180 to 220 g,were randomly divided into 5 groups:control group,and groups of 3,6,24,72 hours after electromagnetic irradiation with 15 rats in each group. INTERVENTIONS:The rats were exposed to microwave with the peak power of 90 W/cm2 for 15 minutes successively,and all the rats survived the irradiation.The serum contents of testosterone of rats in the groups of 3,6,24,72 hours after electromagnetic irradiation and control group were measured by using radioimmunoassay(RIA) respectively;meanwhile the levels of the StAR and P450scc mRNA in testis tissue were also determined with RT-PCR. MAIN OUTCOME MEASURES:Serum contents of testosterone in the groups of 3,6,24,72 hours after electromagnetic irradiation and control group;the expression levels of the StAR and P450scc mRNA in testis tissues. RESULTS:The serum contents of testosterone,the StAR and P45
出处
《中国临床康复》
CSCD
北大核心
2005年第3期229-231,共3页
Chinese Journal of Clinical Rehabilitation
