摘要
目的 观察补肾法对去势兔动脉粥样硬化血清性激素水平及主动脉壁雌激素 -β受体 (ER -β)表达的影响。方法 新西兰雌兔随机分组 :A组 (卵巢切除 )、B组 (未切除卵巢 )、C组 (卵巢切除加补肾煎 )、D组 (卵巢切除加雌激素 ) ,均予高脂饮食加注射牛血清蛋白 ,造成动脉粥样硬化模型。同时C组予补肾煎、D组予雌激素 ,12周后检测相关指标。结果 A组、C组血清雌二醇 (E2 )与B组比较有明显降低 (P <0 .0 5 ) ,而睾酮水平有明显升高 (P <0 .0 5 ) ,D组较A组的血清E2 水平有显著提高 (P <0 .0 1) ;各组兔主动脉均可见到雌激素 -β受体表达 ,B、C组表达较强。结论 补肾法不能直接提高去势兔的血清E2 水平 ,但可提高主动脉壁ER -β的表达 ,从而起到对去势后动脉粥样硬化的防治作用 ,其作用机制有异于激素替代疗法。
Objective To observe the effect of “Kidney-Nourishing Decoction' on hormone level and expression of estrogen receptor-β on aorta wall in ovariectomized rabbits.Methods 40 female New Zealand rabbits were randomized into four groups:group A(OVX),group B(sham operation),group C(OVX with“Kidney-Nourishing Decoction”)and group D(OVX with estrogen),all were established into atherosclerosis rabbit model by feeding high cholesterol diet and injecting bovine serum albumin after ovariotomy.12 weeks later,the related indexes were checked.Results Estradiol level in group A and C are lower than that of group B, while testosterone increased highly. And estradiol level is elevated in group D compared with group A.In the four groups,estrogen receptor-βcan be found ,but its expression are stronger in both group B and C.Conclusion “Kidney-Nourishing Decoction' can not raise the level of estradiol, but could increase the expression of estrogen receptor-βso as to prevent atherosclerosis after ovariotomy,which is different from hormone replacement therapy.
出处
《中西医结合心脑血管病杂志》
2005年第1期31-33,共3页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
基金
国家自然科学基金资助项目 (No .30 2 71 664)
关键词
补肾煎
去势兔动脉粥样硬化
雌二醇
睾酮
雌激素-β受体
kidney-nourishing decoction
ovariectomized atherosclerosis rabbit
estradiol
testosterone
estrogen receptor-β
