摘要
目的 研究中脑导水管周围灰质 (PAG)在偏头痛中的作用。方法 应用大鼠上矢状窦旁硬脑膜刺激模型 ,免疫组化染色及双标染色观察PAG内c Fos表达及其与 5 羟色胺 (5 HT)的共存。结果 5 HT阳性细胞分布主要集中在PAG腹外侧和腹中间区。阳性细胞数头侧至尾侧逐渐增多。c Fos阳性细胞在刺激组比非刺激组和对照组明显增多。双标染色显示 5 HT和c Fos阳性细胞均聚集在PAG腹外侧 ,双标细胞占 5 HT细胞数的 15 0 %± 2 8% ,占c Fos阳性细胞的 2 4 0 %±4 3%。结论 PAG神经元在偏头痛模型中兴奋 ,PAG内 5 HT阳性细胞部分兴奋 ,可能还存在其他类型神经元的兴奋。
Objective To investigate the role of PAG in migraine Methods A transformed superior sinus stimulation migraine model was applied to observe Fos expression and co localization of 5 HT with Fos in PAG following the stimulation of dual matter adjacent to the superior sagittal sinus in rats Results 5 HT immunoreactive neurons, densely located in ventrolateral and ventromedial sectors, were found throughout the PAG Fos like immunoreactivity neurons were obviously increased in stimulated group than in unstimulated and control groups The double labelled neurons accounted for 15 0%±2 8% of 5 HT immunoreactive neurons and 24 0%±4 3% of Fos like immunoreactivity The double labelled population mainly distributed in ventrolateral sector Conclusions It suggests that neurons in PAG, especially in ventrolateral sector in caudal segment, might be excited in migraine model of rat The excited cells and 5 HTnic neurons might have a similar distribution pattern in PAG, with part of excited cells being 5 HTnic neurons Other types of excited neurons and the subtype of 5 HT receptor were not included in the experiment, and would be studied further as to getting a better understanding of the pathophysiology of migraine
出处
《中华神经科杂志》
CAS
CSCD
北大核心
2004年第6期504-506,共3页
Chinese Journal of Neurology
基金
解放军总医院学科带头人工程基金资助项目
