摘要
目的 :观察自体外周血树突状细胞(DC)诱导的淋巴因子激活杀伤 (LAK )细胞对原代肝癌细胞的杀伤活性。方法 :从肝癌患者外周血分离单个核细胞 ,以人粒 /巨噬细胞集落刺激因子 (GM CSF)和白细胞介素(IL ) 4诱导扩增DC ,以四甲基噻唑蓝(MTT )方法观察单独应用IL 2诱导的LAK细胞组和联合应用DC组对肝癌细胞的杀伤活性。结果 :联合应用DC组对肝癌细胞杀伤活性 [( 3 0 76± 1 44 ) % ]明显比单独应用LAK细胞组的活性 [( 18 2±1 2 9) % ]高 ,P <0 0 1。结论 :DC可以提高效应细胞对靶细胞的杀伤活性 。
OBJECTIVE:To study the activity killer of LAK cells against original hepatocellcullar cancer cells induced by autogenous peripheral blood dendritic cells(DC).METHODS:The DC from a hepatocell cancer patient was isolated and purified with combination of granulocyte/macrophage colony stimulating factor and interleukin 4;lymphokine activated killer cells were induced with interleukin 2;the activity killer of LAK cells against original HCC or LAK cells and DC against original HCC was observed by the MTT method.RESULTS:The activity killer of LAK cells and DC against original HCC[(30.76±1.44)%] was higher obviously than that of LAK cells alone[(18.2±1.29)%],P<0.01.CONCLUSTIONS:DC could improve the activity killer of effecting cells resisting target cells,implying that DC may be important in the therapy and prevention against hepatocellcullar cancer.
出处
《肿瘤防治杂志》
2004年第5期499-500,共2页
China Journal of Cancer Prevention and Treatment
关键词
癌
肝细胞/免疫学
癌
肝细胞/治疗
树突细胞
carcinoma,hepatocellular/immunology
carcinoma,hepatocellular/therapy
dendritic cells
