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人胎盘TRAIL基因的cDNA克隆及序列测定 被引量：1

Cloning and sequencing of cDNA of TRAIL from human placenta
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摘要 　根据GeneBank(U37518)中TRAIL(肿瘤坏死因子相关的凋亡诱导配体)的cDNA序列,设计扩增引物。采用RT-PCR从人胎盘中扩增出TRAIL基因的全长cDNA。产物纯化后连至pGEM-TEasy载体,转化大肠杆菌DH5α。筛选阳性克隆菌,酶切鉴定并进行序列测定。结果表明,本试验成功地克隆了TRAIL基因的1039bp全长cDNA。 According to the cDNA sequence of TRAIL (TNF-related apoptosis-inducing ligand,TRAIL) derived from the Genebank (U37518),a pair of primers were designed.RT-PCR was applied to amplify the whole cDNA of TRAIL gene.The DNA fragment was cloned into pGEM-T Easy vector and transformed into E.coli DH5α host bacteria.NotⅠ endonuclease digestion and sequencing of selected positive cloned bacteria were conducted.Result showed the whole 1 039 bp cDNA of TRAIL was successfully cloned.
作者 张明烽 唐爽 宋红卫 张涌
机构地区 西北农林科技大学生物工程研究所
出处 《西北农林科技大学学报（自然科学版）》 CSCD 北大核心 2005年第2期6-8,13,共4页 Journal of Northwest A&F University(Natural Science Edition)
基金 国家"863"高技术资助项目(2001AA213081)
关键词 肿瘤坏死因子相关的凋亡诱导配体(TRAIL) RT-PCR 序列测定 人胎盘 TNF-related apoptosis-inducing ligand (TRAIL) RT-PCR sequencing human placenta
分类号 Q786 [生物学—分子生物学]
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参考文献17

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同被引文献9

  • 1舒茂国,郭树忠,王昕红,郭晓彤,李立文4.人ALK-1的克隆和真核表达载体的构建[J].第四军医大学学报,2005,26(22):2046-2048. 被引量：1
  • 2何晓宁,彭树英,杨瑞锋,刘凤军,郑月茂,张涌.人溶菌酶基因cDNA的克隆和序列分析[J].安徽农业科学,2006,34(11):2333-2335. 被引量：3
  • 3Goumans M J,Lebrin F,Valdmarsdottir G. Controlling the angiogenic switch:A balance between two distinct TGF-b receptor signaling pathways[J]. Trends Cardiovasc Med, 2003, 13 (7):301-307.
  • 4Ozoren N, Kim K,Burns T F, et al. The caspase 9 inhibitor z- LEHD-FMK protects human liver cells while permitting death of cancer cells exposed to tumor necrosis factor-related apoptosis-inducing ligand[J]. Cancer Res, 2000, 60(22): 6259-6265.
  • 5Laeour S, Hammann A, Wotawa A, et al. Antieancer agents sensitize tumor cells to tumor necrosis factor-related apoptosis- inducing ligand-mediated caspase-8 activation and apoptosis[J]. Cancer Res, 2001, 61(4) : 1645-1651.
  • 6Jo M , Kim T H, Seol D W, et al. Apoptosis induced in normal human hepatoeytes by tumor necrosis factor-relatde apoptosis-inducing ligand[J]. Nat Med,2000, 6(5):564-567.
  • 7符莉芳,宋志芳.溶菌酶的研究进展[J].浙江预防医学,2008,20(4):56-59. 被引量：21
  • 8荣晓花,凌沛学.溶菌酶的研究进展[J].中国生化药物杂志,1999,20(6):319-320. 被引量：53
  • 9孙怀昌,张泉,施伟庆,李国才,于峰.人溶菌酶cDNA的克隆及其在小鼠体内的表达[J].中国兽医学报,2004,24(2):157-159. 被引量：19

引证文献1

西北农林科技大学学报（自然科学版）
2005年 第2期

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