摘要
目的 :探讨体外培养的正常兔鼻软骨细胞分离培养方法及生物学特性。方法 :体外分离培养兔鼻软骨细胞 ,观察原代及传代培养的细胞形态学变化 ;测定细胞数量的变化及其生长曲线 ,观察细胞的增殖 ;借助特殊染色 ,免疫组织化学的方法了解葡萄糖胺聚糖 ,Ⅱ型胶原的合成情况 ;测定冷冻复苏后的细胞存活率。结果 :原代体外培养的新西兰白兔兔鼻软骨细胞初始接种时为圆形 ,细胞贴壁后逐渐变为多角形 ;组织学 ,免疫组化显示细胞培养 4代以内可以保持表型的稳定 ;冷冻复苏存活率为 93 %。结论 :体外培养的兔鼻软骨细胞表型能保持 4代稳定 ,具有正常的生长增殖能力 ,并可经深低温冷冻长期保存。
Objective:To study the characteristics of rabbit nasal chondrocytes c ultured in vitro.Method:The rabbit nasal chondrocytes were cult ured in vitro. The changes of cellular morphology from primary to subculture were investigated: to observe the proliferation of chondrocytes by the growth curve ; and to deter mine the biologic synthetic of the chondrocytes by toluidine blue and type Ⅱ co llagen immunohistochemistry reaction;The survive rate of the nasal chondrocytes was observed after being frozen (-196 ℃).Result: The morphology of the primary successfully cultured chondrocytes maintained the spherical shape , and then bec ame polygonal shape. The phenotype of the chondrocytes was maintained in the fir st 4 generation. The survive rate of the chondrocytes was 93%。Conclusio n: The nasal chondrocytes cultured in vitro maintained the specific chondrocytes phenot ype in the first 4 generation , the growth was normal, and could survive in the condition of deep-frozen. [
出处
《临床口腔医学杂志》
2005年第2期86-88,共3页
Journal of Clinical Stomatology
基金
湖南省科技厅基金资助项目 (0 1JZY2 0 1 7)
