摘要
目的 :研究选择性一氧化氮合酶抑制剂氨基胍对脑缺血大鼠脑组织中氨基酸含量的影响 ,探讨氨基胍对脑缺血组织的保护作用及其作用机制。方法 :采用线栓法制备大鼠大脑中动脉缺血模型 ,模型建成后腹腔注射氨基胍。相应时间断头取脑 ,然后测定脑梗死体积、纹状体、海马、皮层中天门冬氨酸、谷氨酸、甘氨酸、γ -氨基丁酸(GABA)含量。结果 :氨基胍组脑梗死体积明显小于缺血组 ;缺血组纹状体、海马、皮层中天门冬氨酸、谷氨酸、甘氨酸、GABA含量显著高于假手术组 ,氨基胍组天门冬氨酸、谷氨酸的含量明显低于缺血组 ,甘氨酸、GABA含量明显高于缺血组。结论 :氨基胍降低脑组织中兴奋性氨基酸的含量 ,升高抑制性氨基酸的含量可能是保护缺血脑组织的重要机制之一。
AIM: By evaluating the effect of selective ni tric oxide synthase inhibitor, aminoguanidine (AG), on the contents of amino aci ds in the rat brain following cerebral ischemia, to investigate the effect of AG on cerebral ischemic injury and the possible mechanism. METHODS: Adult male SD rats, weighing 250-300 g, were matched ra ndomly. We made the focal cerebral ischemic model with thread embolism of left m iddle cerebral artery occlusion (MCAO). AG was administrated after MCAO. Rats we re killed at the scheduled time. The changes of infracted volume and the content s of aspartate, glutamate, glycine and γ-aminobutyric acid (GABA) from striatum , hippocampus and cortex were assayed, respectively. RESULTS: The cerebral infarct volume in AG group were significan tly lower than that in ischemic group. The contents of aspartate, glutamate, gly cine and GABA from striatum, hippocampus and cortex in ischemic group were marke dly higher than those in sham group. The contents of aspartate and glutamate in AG group were significantly lower than those in ischemic group, but the contents of glycine and GABA in AG group were higher than those in ischemic group. CONCLUSION: It may be concluded that AG has a beneficial effect on ischemic rat brain. The possible protective mechanism is that AG can reduce t he contents of aspartate and glutamate, increase the contents of glycine and GAB A.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第2期342-346,共5页
Chinese Journal of Pathophysiology
基金
河北省自然科学基金资助课题 (No .398330 )
关键词
脑缺血
一氧化氮
一氧化氮合酶
氨基胍
氨基酸
Brain ischemia
Nitric oxide
Nitric-oxide sy nthase
Aminoguanidine
Amino acid
