摘要
目的建立海蟑螂中药理作用成分腺苷的含量测定方法。方法海蟑螂全体经37℃水温浸提取,硅胶 薄层层析分离,用紫外分光光度法于260nm处测定腺苷吸光度。结果在1.0～20.0μg·mL-1的范围内腺苷 的浓度和吸光度呈良好线性关系(r=0.9998,n=6),平均回收率为99.3%(RSD=1.9%,n=3)。结论本法 简捷、准确,适用于海蟑螂中腺苷的含量测定。
Objective To establish a UV method of determination of adenosine in Ligia exotica. Methods Adenosine was extracted with 37℃ water and separated by TLC silica gel, the absorbance was detected at 260nm with UV spectrophotometry.Results The cabibration curve for adenosion was linear within the range of 1.0~20.0μg·mL -1, r=0.9998 (n=6), the average recovery was 99.3%,RSD=1.9% (n=3). Conclusion The method was simple, convenient and accurate for determination of adenosine in Ligia exotica.
出处
《中国海洋药物》
CAS
CSCD
2005年第1期40-42,共3页
Chinese Journal of Marine Drugs
关键词
海蟑螂
腺苷
紫外分光光度法
含量测定
Ligia exotica
adenosine
ultraviolet spectrophotometry
assay
