摘要
探讨不同浓度的维生素C(AscorbicAcid ,Asc)对培养大鼠肋生长板软骨细胞 (ratcostochondralgrowthplatechondrocyte,RGC)增殖和胶原合成的影响。分离、培养RGC ,以组织化学和免疫组织化学的方法鉴定第 2代RGC的表型 ;分别用3 H TdR和3 H Proline掺入法检测 2 5、5 0和 10 0mg/LAsc对第 2代RGC增殖和胶原合成的影响。3种浓度的Asc均能促进RGC胶原合成 (P <0 0 5 ) ,2 5mg/L和 5 0mg/LAsc的促胶原合成作用明显强于 10 0mg/L(P <0 0 5 ) ;2 5mg/L和 5 0mg/L的Asc促进RGC增殖 (P <0 0 5 ) ,10 0mg/L的Asc抑制RGC的增殖 (P <0 0 5 )。因此一定浓度的Asc具有促进RGC增殖和胶原合成的作用 ,2 5mg/L～ 5 0mg/L是较为合适的添加浓度。
To study the effects of Ascorbic acid (Asc)on proliferation and collagen synthesis of the rat costochondral growth plate chondrocyte (RGC). Cultured RGC and were examined for the proteoglycan and collagen Ⅱexpressed by the second passage chondrocyte with histochemistry and ICC, the effects of 25, 50 and 100 mg/L Asc on their proliferation and collagen synthesis was tested by 3 H-TdR and 3 H-Proline incorporation. The second passage RGC expressing collagen Ⅱand proteoglycan retained their in vivo phenotype. All the three concentrations of Asc promoted collagen synthesis (P<0.05), the effects of 25 mg/L and 50 mg/L were significantly higher than 100 mg/L (P<0.05); in addition, 25 mg/L and 50 mg/L Asc stimulated RGC proliferation (P<0.05), but 100 mg/L Asc inhibited RGC proliferation significantly (P<0.05). Ascorbic acid facilitates collagen synthesis and proliferation of cultured RGC.
出处
《基础医学与临床》
CSCD
北大核心
2004年第6期629-632,共4页
Basic and Clinical Medicine
基金
973国家重点基础研究发展规划项目 (G19990 5 4 30 3)
