摘要
人膀胱癌相关蛋白 (BLCAP)基因是本研究室从细胞原癌、抑癌基因分类芯片中筛选并克隆的宫颈癌候选抑癌基因。为检测该基因调控区的单核苷酸多态性 (SNP)及其不同基因型与宫颈癌的相关性 ,采用病例 对照研究方法及动态等位基因杂交 (DASH)技术 ,对 30例原发性宫颈癌患者和 6 0例正常人BLCAP基因调控区的 2个SNP位点进行了检测。结果显示 :位于BLCAP基因下游调控区的SNPrs3795 14 7位点存在AA、AC和CC 3种基因型 ,且与宫颈癌发病存在显著相关性 (P <0 0 1)。SNPrs3795 14 7处在BLCAP基因下游调控区域 ,其多态类型可能在某种程度上影响BLCAP基因的表达调控 ,从而进一步支持了 :BLCAP基因与宫颈癌的发生、发展可能存在密切关系。
To analyze the cervical carcinoma specimen with an oncogene and anti-oncogene cDNA microarray. We found that Homo sapiens bladder cancer associated protein(BLCAP) gene down-expressed. After a series of experiments, we ascertained that BLCAP gene was a candidate tumor suppressor gene for cervical carcinoma. In order to definitude the function of BLCAP gene in the development of human cervical carcinoma, we studied two single nucleotide polymorphisms (SNPs) within BLCAP gene in this experiment. We analysed 30 unrelated cervical carcinoma cases and 60 control subjects, which matched to the cervical carcinoma cases in age and residence, by means of dynamic allele specific hybridization (DASH). Significant results were obtained from one SNP site (rs3795147), which was located at 711bp down-stream of BLCAP gene. SNP rs3795147 had three kinds of genotype:AA,AC and CC, and results of χ 2 test showed that the frequencies of different genotypes resulted in cervical carcinoma cases and control subjects were significantly different(P<0.01). The result proves again that BLCAP gene plays a certain role in the development of cervical carcinoma, for the SNP site rs3795147 may influence the expression of BLCAP gene.
出处
《基础医学与临床》
CSCD
北大核心
2004年第6期646-650,共5页
Basic and Clinical Medicine
基金
湖北省自然科学基金 (30 1130 735 )
