摘要
目的探讨原花青素(procyanidins,PC)对β淀粉样肽(2535)〔βamyloidpeptide(2535),Aβ2535〕诱导PC12细胞凋亡的保护作用。方法采用MTT比色法分析细胞存活率,Hoechst33258PI荧光染色法检测凋亡,流式细胞仪检测分析细胞凋亡率变化情况。结果不同剂量PC预处理PC12细胞1h可剂量依赖性对抗Aβ2535引起的PC12细胞凋亡,提高细胞的存活率,减少Aβ2535引起的核固缩、凝聚和碎裂,降低细胞凋亡率。结论PC可剂量依赖性对抗Aβ2535对PC12细胞的毒性作用。
Objective To explore the protective effect of procyanidins(PC)on apoptosis of PC12 cells induced by β-amyloid peptide 25-35(Aβ_~25-35 ). Methods Cell survival rate was measured by MTT assay and apoptosis was detected by Hoechst-PI fluorescence staining and flow cytometry. Results Pretreatment with PC of different concentrations for 1 h could prevent PC12 cells against apoptosis induced by Aβ_~25-35 and increase the survival rate of PC12 cells in a dose-dependent fashion. PC prevented the PC12 cells nuclei from shrinkage, condensation and cleavage induced by Aβ_~25-35 and decreased its apoptosis rate. Conclusions PC can resist the toxic effect of Aβ_~25-35 on PC12 cells in a dose-dependent manner.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2005年第2期159-161,共3页
Chinese Journal of Gerontology
基金
广东省重点学科重点项目(9808)
关键词
原花青素
AΒ25-35
PC12细胞
凋亡
Procyanidins (PC)
β-amyloid peptide (Aβ_(25-35) )
PC12 cells
Apoptosis
