摘要
研究弓形虫感染对小鼠妊娠的影响。用免疫组织化学方法测定子宫部分免疫细胞,用酶联免疫吸附法测定子宫匀浆中肿瘤坏死因子 α(TNF α)和白介素 10(IL 10)的含量。发现孕0天和孕6天接种弓形虫,小鼠全部流产。感染组子宫内膜中仅见少量CD8+T淋巴细胞。对照组未见阳性细胞。F4/80+巨噬细胞在感染组小鼠子宫内膜中大量存在。对照组仅见少量F4/80+细胞。细胞因子测定结果表明,TNF α弓形虫感染组子宫匀浆中含量高达76 862±30 354pg/mg蛋白,显著高于正常妊娠7天时小鼠子宫中TNF α的含量(P<0 01)。结果提示,已妊娠母鼠感染弓形虫可引发流产,并与子宫胎儿界面的TNF α分泌增多有关。孕0天感染,孕鼠子宫匀浆上清液中IL 10含量平均值为19 696±9 811pg/mg蛋白,显著低于对照组(48 856±16 518pg/mg,P<0 05)。孕6天感染,子宫匀浆IL 10含量与对照组比较差异不显著(P>0 05)。
The influence of Toxoplasma gondii infection on the success of mice pregnancy was investigated in this paper. Immunohistochemistry was applied to locate immunocytes in the uterus, and enzyme-linked immunosorbent assay(ELISA) was conducted in order to determine the contents of tumor necrosis factor-α(TNF-α) and interleukin-10 in uterine lysates. All the mice were aborted when the Toxoplasma gondii were incubated intraperitoneously on day 0 or day 6 of pregnancy. A few CD8^+T lymphocytes were scattered in the endometrium of infected animals, while none cells were seen in the control mice. Large number of F4/80^+ macrophages were counted in the infected mice. A few were seen in the control group. (TNF-α) level was significantly higher(76.862±30.354 pg/mg protein) in the infected mice compared with the control animals(P<0.01),indicating a close correlation between pregnancy failure and Toxoplasma infection. IL-10 contents were 19.696±9.811 pg/mg protein in the supernatants of the mice infected with Toxoplasma on day 0, significantly lower than those of control group(48.856±16.518 pg/mg protein,P<0.05). No significant differences of IL-10 contents were observed between the infected mice and controls when infection was carried out on day 6 of pregnancy (P>0.05).
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2005年第1期77-81,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(No.30270980)
河北省畜牧局项目(2001 5)
