摘要
目的建立大鼠血浆中游离脂肪酸 (FFAs)浓度的测定方法。方法以十七烷酸为内标 ,用 ψ(异丙醇∶正庚烷∶2mol/L磷酸 ) =(4 0∶10∶1)提取 ,溴苯乙酮衍生化 ,流动相为 ψ(乙腈∶水 ) =(81∶19) ,流速 1.0mL/min ,柱温 5 5℃ ,分析柱Nova pakC18(2 5 0mm× 4 .6mm ,4 μm) ,紫外检测波长 2 4 2nm。 结果各FFAs最低检测限为 0 .6 μmol/L ,平均回收率为 (10 0 .1± 2 0 ) % ,批内、批外RSD <6 .5 %。结论本法稳定、灵敏、可靠 。
Objective To develop a HPLC method for determination of free fat acids(FFAs) in rat plasma. Methods Using heptadecanoic acid as the internal standard, FFAs were extracted from rat plasma with a mixture of isopropanol∶heptane∶ phosphoric acid (2 mol/L)=40∶10∶1. FFAs were derived by phenacyl bromide. The mobile phase was acetonitrile∶water(81∶19) at 1.0 mL/min with UV detection at 242 nm and column temperature was 55 ℃. The analytic column was Nova-PAK C 18(250 mm×4.6 mm, 4 μm). Results The limit of detection was 0.6 μmol/L. The average recovery was (100.1±2.0)%, and the relative standard deviation of intraday and interday were limited within 6.5%, respectively. Conclusion The method was stable, sensitive and accurate, and suitable for determination of FFAs in rat plasma.
出处
《广东药学院学报》
CAS
2004年第6期656-658,共3页
Academic Journal of Guangdong College of Pharmacy
