摘要
目的 :探讨饮用氟水大鼠骨RNA和ROS的含量变化在氟骨症早期发病机理中的作用。方法 :Wistar大鼠80只 ,雌、雄各半 ,平均体重为 90 g± 5 g。随机分成 4组 ,用试剂盒测定血清中丙二醛 (MDA)及骨组织中的RNA的含量。用ESR检测骨ROS的含量。结果 :低、中、高氟组骨ROS及血清中MDA含量变化含量均有所升高 (F =3.98,P <0 .0 1) ,骨中RNA含量随氟剂量的增加而逐渐减少。结论 :提示饮用水中的氟含量会影响体内脂质过氧化物水平升高降低 。
Objective: To investigate the role of ROS and RNA content in rat drinking fluoride water on the pathogenesis of skeletal fluorosis.Methods: 80 Wister rats with the average body-weight 90g±5g were divided randomly into four groups. MDA in serum and RNA in bone were determined by reagent box to detect ROS in bone by ESR. Results: Bone ROS and serum MDA in low、middle、high fluoride groups increased obviously(F=3.98,P<0.01), bone RNA decreased with fluoride content(F=3.98,P<0.01).Conclusion: The results indicate that the fluoride contents in drinking water may influence ROS content in bone , which affects RNA synthesis ability of bone .
关键词
氟骨症
自由基
影响
skeletal fluorosis
free radical
effect
