摘要
目的 利用我们以往建立的便秘型肠易激综合征(C-IBS)大鼠模型,对该模型中SP、VIP、GABA、Ach、NE在肠神经系统的分布及表达进行研究,探讨这些物质在IBS发病机制中的可能作用。方法 采用以往本研究小组建立的C-IBS大鼠模型,取模型组及正常对照组大鼠的回盲部、结肠标本,进行常规组织固定、包埋、切片。分别应用抗SP、VIP、GABA、乙酰胆碱酯酶(AchE)、酪氨酸羟化酶(TH)抗体进行免疫组织化学染色,结果应用彩色病理图像分析系统对阳性表达的面积、不透光率密度值进行半定量分析。结果 C-IBS大鼠模型回盲部、结肠肌层中SP表达的不透光率密度明显增高(P<0.05),阳性面积与正常对照组无显著差异(P>0.05);C-IBS大鼠模型回盲部、结肠肌层中VIP表达的阳性面积、不透光率密度均明显增高(P<0.05);C-IBS大鼠模型回盲部、结肠肌层中GABA表达的不透光率密度明显降低(P<0.05),阳性面积与正常对照组无显著差异(P>0.05);C-IBS大鼠模型回盲部、结肠肌层中AchE、TH表达的阳性面积、不透光率密度与正常对照组尤显著差异(P>0.05)。结论 C-IBS大鼠模型中肠神经系统SP、VIP、GABA表达具有改变,提示这些神经递质在肠道可能参与C-IBS的病理生理过程。
Objective To investigate the expression of the neurotransmitters in the enteric nervous system (ENS) of the rat C-IBS model and to explore their possible role in the pathogenesis of IBS. Methods The tissue specimens were obtained from the rats of control group and the C-IBS model, which was set up and reported by our group. The specimens of the ileocecal junction and colon were stained by immunohistochemical staining using anti - SP, VIP, GABA, acetylcholinesterase (AchE) and tyrosine hydroxylase (TH) antibodies respectively. The staining results were analyzed semi-quantitatively by computerized color image analyzer using the following parameters: immunoreactive area, Opacity density. Results Opacity density of SP immunoreactive tissues in the model group were significantly higher than those in control group (P < 0.05), meanwhile there were no difference between the two groups concerning the immunoreactive area ( P > 0.05) ; immunoreactive area and opacity density of VIP immunoreactive tissues in the model group were all significantly higher than those in control group( P < 0.05); opacity density of GABA immunoreactive tissues in the model group were significantly lower than those in control group ( P < 0.05) , meanwhile there were no differences between the two groups concerning the immunoreactive area ( P > 0.05); there were no differences between the two groups concerning the immunoreactive area, opacity density of Ache and TH immunoreactive tissues (P > 0.05). Conclusion The expressions of SP, VIP, GABA in the ENS in the rat model of C-IBS were abnormal, which suggests that SP, VIP, GABA may play some roles in bowel in the pathogenesis of IBS.
出处
《胃肠病学和肝病学杂志》
CAS
2004年第6期578-583,共6页
Chinese Journal of Gastroenterology and Hepatology
关键词
大鼠模型
IBS
表达
回盲部
结肠
肠神经系统
对照组
GABA
神经递质
中肠
Irritable bowel syndrome
Rat
Substance P
Vasoactive intestinal peptide
Gamma-aminobutyric acid
Acetylcholine
Norepinephrine
