摘要
目的 研究小鼠免疫接种基因重组乙型肝炎表面抗原疫苗(recombinant hepatitis B virus(HBV)surface vaccine,rHBs)产生的特异性淋巴细胞增殖反应。方法 40只BALB／c小鼠随机分为0.65、1.25、2.5、5 μg四组,腹腔分别接种0.65、1.25、2.5、5 μg的rHBs,一半4、鼠2周后加强免疫1次。分别在初次免疫后2周或加强免疫后2周分离小鼠脾T淋巴细胞;分别进行以下实验:实验组用rHBs(10 μg／m1)刺激脾T淋巴细胞,对照组用PBS代替rHBs刺激脾T淋巴细胞;3天后用3H掺入法检测脾T淋巴细胞特异性增殖反应,以3H掺入的同位素cpm值及刺激指数(Stimulation index,SI,实验组cpm值／对照组cpm值)表示。结果 只接受单次免疫的0.65,1.25,2.5,5 μg组小鼠脾T淋巴细胞特异性增殖反应SI分别为1.55、1.93、2.41、2.81;而接受加强免疫的0.65、1.25、2.5、5 μg组小鼠脾T淋巴细胞特异性增殖反应SI分别为1.61、2.05、3.74、3.62。结论 小鼠接种rHBs后产生特异性淋巴细胞增殖反应,加强免疫或增加接种剂量可以增强反应强度。
Objective To evaluate the immunogenicity of mice with recombinant hepatitis B virus (HBV) surface vaccine (rHBs) by lymphocyte proliferation. Methods BALB/c mouse in 4 groups were vaccinated intraperitoneally with rHBs 0.65,1.25,2.5 and 5 μg respectively Four jection, spleen lymphocytes were restimulated with rHBs in vitro in experimental group or PBS in control group. H-thymidine incorporation was measured after 3 days. Results Two weeks after primary vaccination, the stimulation index, SI in 0. 65 , 1 . 25 , 2. 5 and 5 μg group were 1. 55 , 1. 93 , 2.41 and 2.81 respectively. 2 weeks after boost vaccination, the SI in 0.65 ,1.25 ,2.5 and 5 μg group were 1.61,2 .05 ,3 .74 and 3 .62 respectively. Conclusion Vaccination with rHBs result in specific proliferative response. The strengthening of T-lymphocyte proliferation of mouse vaccinated with rHBs is associated with the dose of vaccination or boost vaccination.
出处
《胃肠病学和肝病学杂志》
CAS
2004年第6期592-594,共3页
Chinese Journal of Gastroenterology and Hepatology
基金
广州市重点科技项目(99M04811G)
