期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

缬草波春联合Caspases抑制剂诱导MKN45胃癌细胞凋亡 被引量：2

Valepotriate combined with Caspases inhibitors induced apoptosis of human gastric cancer cell line MKN45
下载PDF
导出
摘要 目的 研究中草药缬草提取物缬草波春联合Caspases抑制剂诱导MKN45胃癌细胞凋亡的作用。方法 将MKN45胃癌细胞分成4组:第1组加入同体积的生理盐水为对照;第2组分别加入浓度为10μM的Caspases-3、-8、-9抑制剂;第3组加入浓度为100 mg／L的缬草波春;第4组加入浓度为10 μM的Caspases-3、-8、-9抑制剂+浓度为100 mg／L的缬草波春混合物。药物作用24小时、48小时、72小时后,用流式细胞计分别检测凋亡率。结果　24、48、72小时作用后,第2组诱导MKN45细胞的凋亡率与对照组相似(P>0.05);第3组诱导MKN45细胞的凋亡率明显高于照组相(P<0.01);第4组中缬革波春联合Caspases-3、-9抑制剂诱导MKN45细胞的凋亡率均与对照组相似(P>0.05);缬草波存与Caspases-8抑制剂联合应用组诱导MKN45细胞的凋亡率明显高于对照组比(P<0.01),与第2组相似(P>0.05)。结论 缬草波春能诱导MKN45胃癌细胞凋亡,诱导凋亡能被Caspases-3、-9抑制剂抑制,Casoases-8抑制剂对缬草波春诱导的调亡无影响。 Objective To study the apotosis of human gastric cancer cell line MKN45 induced by Valepotriate combined with Caspases inhibitors. Methods Human gastric cancer cell line MKN45 cells were divided into 4 groups: control group (Group Ⅰ), MKN45 cells were exposed to 10 μM of Caspases-3, -8, -9 inhibitors for 24, 48, 72 hours, respectively (Group Ⅱ); MKN45 cells were exposed to 100mg/L of Valepotriate blending solution for 24, 48, 72 h ours, respectively (Group Ⅲ); MKN45 cells were exposed to 10 μM of Caspases-3, -8, -9 inhibitors combined with 100mg/L of Valepotriate for 24,48,72 hours, respectively (Group Ⅳ) .Induced apoptosis rate of human gastric cell cancer line MKN45 was detected by FACS . Results After 24, 48 , 72 hours exposure, induced apoptotic rates of human gastric cancer cell line MKN45 in Group Ⅱ were similar to that of Group Ⅰ (P > 0.05), induced apoptotic rate in Group Ⅲ was much higher than that of Group Ⅰ ( P < 0.01), apoptotic rates induced by Valepotriate combined with Caspase-3 and Caspase-9 inhibitors in Group Ⅳ were similar to that of Group Ⅰ ( P > 0.05), apoptotic rates induced by Valepotriate combined with Caspase-8 inhibitor in Group IV was similar to that of Group Ⅱ ( P > 0.05) and was much high than that of Group Ⅰ ( P < 0.01) . Conclusion These data indicated that apoptosis of human gastric cancer cell line MKN45 can be induced by Valepotriate, which can be inhibitored by cas pases- 3 inhibitor and caspases- 9 inhibitor, not by caspases- 8 inhibitor.
作者 叶建明 易粹琼 薛存宽 钱伟 呼闯营 陈凤鸣
机构地区 华中科技大学同济医学附属协和医院消化内科 华中科技大学同济医院老年医学研究所 华中科技大学同济医学附属协和N院消化内科
出处 《胃肠病学和肝病学杂志》 CAS 2004年第6期619-621,共3页 Chinese Journal of Gastroenterology and Hepatology
关键词 MKN45 缬草 诱导 凋亡率 抑制剂 胃癌细胞凋亡 联合 流式细胞计 体积 Valepotriate Gastric neoplasms Apoptosis Caspases
分类号 R735 [医药卫生—肿瘤] R284.1 [医药卫生—中药学]
  • 相关文献

参考文献7

  • 1Hadley S, Petry JJ. Valerian. Am Fam Physician. 2003 ;67(8): 1755-1758.
  • 2Bounthanh C, Bergmann C, Beck JP, et al. Valepotriates, a new class of cytotoxic and antitumor agents. Planta Med, 1981,41 (1): 21-28.
  • 3Bounthanh G, Richert L, Beck J. P, et al. The action of Volepotriates on the Algnthesis of DNA and Proteins of cultured hepatoma cells. Planta Med,1983,49(3): 138-142.
  • 4Keochanthala-Bounthanh C, Haag-Berrurier, M, Beck JP, et al. Effects of thiol compounds versus the cytotoxicity of valepotriates on cultured hepatoma cells. Planta Med. 1990;56(2): 190-192.
  • 5Verhagen AM, Coulson EJ , Vaux DL linhibitor of apoptosis proteins and their relatives:IAPS and other BIRPs. Genome Biol. 2001;2(7): reviewS3009.
  • 6徐军美,胡冬煦,常业恬.Caspases抑制剂抑制细胞凋亡研究进展[J].国外医学（生理病理科学与临床分册）,2002,22(3):254-256. 被引量：2
  • 7吕翠仙,樊廷俊,胡国斌,丛日山.凋亡诱导因子与细胞凋亡(英文)[J].生物化学与生物物理学报,2003,35(10):881-885. 被引量：5

二级参考文献32

  • 1FanTJ XiaL HanYR.Mitochondrion and apoptosis[J].Acta Biochimica et Biophysica Sinica(生物化学与生物物理学报:英文版),2001,33(1):7-12.
  • 2Cande C, Cecconi F, Dessen P, Kroemer G. Apoptosis-inducing factor (AIF): Key to the conserved caspase-independent pathways of cell death? Journal of Cell Science, 2002, 115(24): 4727 -4734.
  • 3Cande C, Cohen I, Daugas E, Ravagnan L, Larochette N, Zamzami N, Kroemer G. Apoptosis-inducing factor (AIF): A novel caspaseindependent death effector released from mitochondria. Biochimie, 2002, 84(2-3): 215 -222.
  • 4van Gurp M, Festjens N, van Loo G, Saelens X, Vandenabeele P. Mitochondrial intermembrane proteins in cell death. Biochem Biophys Res Commun, 2003, 304(3): 487 -497.
  • 5Tsujimoto Y. Cell death regulation by the Bcl-2 protein family in the mitochondria. J Cell Physiol, 2003, 195(2): 158 - 167.
  • 6Arnoutt D, Parone P, Martinou JC, Antonsson B, Estaquier J, Ameisen JC. Mitochondrial release of apoptosis-inducing factor occurs downstream of cytochrome c release in response to several proapoptotic stimuli. J Cell Biol, 2002, 159(6): 923 -929.
  • 7Mate MJ, Ortiz-Lombardia M, Boitel B, Haouz A, Tello D, Susan S. A, Penninger J et al. The crystal structure of the mouse apoptosisinducing factor (AIF). Nat Struct Biol, 2002, 9(6): 442 -446.
  • 8Wu M, Xu L, Li X, Zhai Z, Shu H. AMID, an apoptosis-inducing factor-homologous mitochondrion-associated protein induces caspase-independent apoptosis. Biol Chem, 2002, 277(28): 25617 -25623.
  • 9Ohiro Y, Garkavtsev I, Kobayashi S, Sreekumar KR, Nantz R, Higashikubo BT, Duffy SL et al. A novel p53-inducible apoptogenic gene, PRG3, encodes a homologue of the apoptosis-inducing factor (AIF). FEBS Lett, 2002, 524(1-3): 163 - 171.
  • 10Cai J, Yang J, Jones DP. Mitochondrial control of apoptosis: The role of cytochrome c. Biochim Biophys Acta, 1998, 1366(1-2): 139- 149.

共引文献5

同被引文献34

  • 1叶建明,易粹琼,薛存宽,呼闯营,陈凤鸣,钱伟.缬草波春诱导胃癌细胞凋亡与信号分子表达的关系[J].中华消化杂志,2004,24(10):619-620. 被引量：6
  • 2薛存宽,何学斌,张书勤,黄晓桃.缬草环烯醚萜抗肿瘤作用的实验研究[J].现代中西医结合杂志,2005,14(15):1969-1972. 被引量：23
  • 3叶建明,胡品津,易粹琼,薛存宽,呼闯营,陈凤鸣,钱伟.缬草波春诱导MKN-45胃癌细胞凋亡[J].世界华人消化杂志,2007,15(1):22-28. 被引量：9
  • 4Piccinelli AL, Arana S, Caceres A, di Villa Bianca R, Sorrentino R, Rastrelli L. New lignans from the roots of Valeriana prionophylla with antioxidative and vasorelaxant activities. J Nat Prod 2004; 67: 1135-1140.
  • 5Fernandez S, Wasowski C, Paladini AC, Marder M. Sedative and sleep-enhancing properties of linarin, a flavonoid-isolated from Valeriana officinalis. Pharmacol Biochem Behav 2004; 77:399-404.
  • 6Wagner H, Jurcic K. [On the spasmolytic activity of valeriana extracts (author's transl)] Planta Med 1979; 37:84-86.
  • 7Malva JO, Santos S, Macedo T. Neuroprotective properties of Valeriana officinalis extracts. Neurotox Res 2004; 6:131-140.
  • 8Eadie MJ. Could valerian have been the first anticonvulsant? Epilepsia 2004; 45:1338-1343.
  • 9Trapet P. [Antidepressants. Principles and rules of use] Rev Prat 1995. 45:.1696-1700.
  • 10Bourdu S, Dapoigny M, Chapuy E, Artigue F, Vasson MP, Dechelotte P, Bommelaer G, Eschalier A, Ardid D. Rectal instillation of butyrate provides a novel clinically relevant model of noninflammatory colonic hypersensitivity in rats. Gastroenterology 2005; 128:1996-2008.

引证文献2

二级引证文献4

胃肠病学和肝病学杂志
2004年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部