摘要
目的 探讨凋亡相关基因caspase-9和Bcl-2在大肠腺瘤、大肠癌中的表达及意义。方法 应用免疫组化S-P法检测56例大肠癌、48例大肠腺瘤及20例正常大肠黏膜中的caspase-9和Bcl-2蛋白的表达。用TUNEL法检测细胞调亡。结果 正常大肠黏膜、大肠腺瘤和大肠癌中caspase-9的阳性表达率分别为5.00％、33.33％、64.29％,其表达率在三者间有显著性差异(P<0.01)。Bcl-2在正常大肠黏膜、大肠腺瘤和大肠癌中的阳性表达率分别为15.00％、87.50％、60.71％,三者间亦有显著性差异(P<0.01)。caspase-9,Bcl-2的表达与肿瘤的分化程度有关(P<0.01),与Dukes分期无关(P>0.05)。正常大肠黏膜、腺瘤和大肠癌中凋亡指数有显著性差异(P<0.01),凋亡指数与肿瘤的分化程度有关(P<0.01),与Dukes分期无关(P>0.05)。caspase-9,Bel-2的表达与凋亡指数有密切联系(P<0.01)。结论 在大肠癌形成过程中,caspase-9,Bcl-2参与了肿瘤形成的共同通道;肿瘤早期阶段细胞凋亡的异常和增生过度,楞能是大肠癌的发病原因之一。
Objective To investigate the expression of apoptosis-related gene caspase-9, Bcl-2 and their clinical significances in colorectal adenoma. Methods Expressions of caspase-9, Bcl-2 were detected in 56 cases of colorectal carcinomas, 48 cases of colorectal adenomas and 20 cases of normal tissues by immuohistochemical method. Apoptotic cells were detected by TUNEL method. Results Positivity of caspase-9 was 5.00% of normal colorectal mucosas,in 33.33% of adenomas and in 64.29% of cancer specimens. Positivity of Bcl-2 15.00% , 87.50% and 60.71% respectively. There was significiant difference in normal colorectal mucosas,adenomas and carcinomas( P < 0.01) .The difference of apoptosis index (AI) was significant in normal eolorectal mucosas,adenomas and carcinomas( P < 0.01) .The expressions of caspase-9 and Bcl-2 were reversely correlated to AI( P < 0.01) .Conclusion There is high expression of caspase-9 and low expression of Bcl-2 in human colorectal carcinoma. The results suggest that the abnormality of apoptosis and the augmentation of proliferation are the possible mechanism of the colorectal carcinogenesis.
出处
《胃肠病学和肝病学杂志》
CAS
2004年第6期639-641,共3页
Chinese Journal of Gastroenterology and Hepatology
