摘要
目的探讨柯萨奇病毒和腺病毒受体(coxsackievirusandadenovirusreceptor,CAR)在嗜心性柯萨奇B3病毒(myocarditiccoxsaekievirusB3,CVB3m)感染心肌细胞中的作用。方法采用体外培养新生小鼠心肌细胞方法,建立CVB3m感染心肌细胞模型,将培养48h的心肌细胞分为对照组、CVB3m组和CAR抗体+CVB3m组,并作相应处理,继续培养48h后,分别观察各组心肌细胞病变效应(cytopathiceffect,CPE),并用四唑盐(MTT)比色法测定各组心肌细胞存活力。结果CAR抗体+CVB3m组心肌细胞CPE明显减轻,存活力显著增强,与CVB3m组比较差异显著(P<0.01),与对照组比较差异无显著性(P>0.05)。结论CAR抗体对CVB3m感染心肌细胞具有保护作用,提示CAR在CVB3m感染心肌细胞中可能发挥重要的介导作用。
Objective To explore the role of coxsackievirus and adenovirus receptor (CAR) on toxie cardiomy-ocytes infected by myocarditic coxsackievirus B3 (CVB3m).Methods A toxic cellular model was established in vitro by adding CVB3m into the culture of neonatal mouse cardiomyocytes.48 hours after co- culturing, the cardiomyocytes were divided into control,CVB3m and CAR antibody+CVB3m groups and processed correspondently.CVB3m- mediated myocyto-pathic effects on these three groups were observed after further co-culturing for 48 hours.At the same time,the cardiomyocytes' viability of these three groups was assessed with MTT assay.Results The degree of cytopathic effect (CPE) in CAR antibody +CVB3m group decreased significantly compared with in CVB3m group(P<0.01) while significant increase of cell viability in CAR antibody+CVB3m group in comparison with in CVB3m group(P<0.01 ).No significant differences was found between CAR antibody+CVB3m group and control group (P>0.05).Conclusions CAR antibody has a protective effect on toxic cardiomyocytes infected by CVB3m, indicating that CAR may play an important role in mediating cardiotoxicity infected by CVB3m.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2004年第11期748-750,753,共4页
Journal of Clinical Pediatrics
关键词
柯萨奇病毒和腺病毒受体
柯萨奇B3病毒
心肌细胞
小鼠
coxsackievirus and adenovirus receptor (CAR) coxsackievirus B3 (CVB3) cardiomyocyte mouse
