摘要
将非标记抗体技术应用于流行性出血热病毒抗原研究,对感染细胞内病毒抗原及经免疫转印到硝酸纤维膜上的病毒多肽抗原进行了检测,并分别与间接免疫荧光和间接酶标抗体方法进行了比较。结果表明,非标记抗体技术敏感性高,背景浅,可用于流行性出血热病毒抗原检测与分析。用非标记抗体技术及间接免疫荧光法对流行性出血热单克隆抗体培养上清液进行筛选,两种方法阳性检出率相同(7.29％,7/96),但前者滴度(1:16～256)高于后者(1:4～32)。
Epidemic Hemorrhagic Fever (EHF) virus antigen in the infected Vero-E6 cells and the virus polypeptide antigen transferred to nitrocellulose membrane were detected by unlabelled antibody technique using self - prepared peroxidase-antiperoxidase (PAP) complex and compared with IFA test and indirect immunoperoxidase (IIP) method. The results showed that the unlabelled antibody technique had a higher sensitivity and a slighter background staining intensity than IFA and IIP. Furthermore, the unlabelled antibody technique also has been used to screen the monoclonal antibody against EHF virus in culture supernatant. The positive rate by PAP method was concordant with that by IFA (7. 29%, 7/ 96), but the former assay showed higher antibody litres (1: 16 - 256) than the latter (1: 4 -32).
出处
《同济医科大学学报》
CSCD
北大核心
1993年第5期299-301,共3页
Acta Universitatis Medicinae Tongji
关键词
非标记
抗体技术
流行性出血热
unlabelled antibody technique
hemorrhagic fever, epidemic
antigens. virus
