摘要
目的:探讨空肠弯曲菌28-31 ku外膜蛋白的免疫原性. 方法:将BALB/c小鼠分为正常对照组和不同剂量抗原的免疫组,正常对照组不予抗原免疫,而免疫组采用28- 31 ku蛋白(纯)或甘氨酸提取的外膜蛋白(粗),分别加用或不加用完全弗氏佐剂(F),采用sc,在0,1,2,3, 4 wk免疫,于末次免疫后10 d,用双向免疫琼脂扩散试验测定各免疫组抗血清效价,待效价达到1:4至1:16 时.分别采集各免疫组及对照组小鼠的血清、空肠及回肠内的肠液,用间接ELISA法检测各组标本的特异性抗体IgA,IgG. 结果:不同剂量的抗原sc免疫BALB/c小鼠后,免疫组血清及肠液中特异性IgA,IgG抗体水平较正常对照组及实验对照组显著升高(P<0.05). 结论:空肠弯曲菌的28-31 ku外膜蛋白是一种良好的免疫原,能够诱导BALB/c小鼠产生特异性抗体,可能成为空肠弯曲菌亚单位疫苗候选的重要组分.
AIM: To explore immunogenicity of 28-31 ku outer membrance protein of Campylobacter jejuni(C.jejuni) and to pave the way for preparation of Cjejuni subunit vaccine. METHODS: BALB/c mice were divided into normal control group, control group and immune group. Mice in immune group were subcutaneously (SC) injected 28-31 ku protein (Pur) or glycine extraction (A), adding or without adding the complete Freud's adjuvant (F). Mice in control group were treated with F and saline. Each mouse received five times of immunization at an interval of 6 days. At 10th day after the final immunization, the specific antibodies' titer in serum was measured with double immunodiffusion test. ELISA method was used to detect the specific IgG, IgA antibodies in serum and intestinal fluid. RESULTS: After treated with different doses of antigen, the levels of specific IgG, IgA or secretory IgA (SIgA) antibodies in serum and intestinal fluid were significantly higher in immune group than those in normal control or control group (P<0.05). No significant difference existed between normal control and control group. CONCLUSION: Outer membrane protein of C.jejuni with molecular mass of 28-31 ku is a fine immunogen and it can induce response of specific antibodiesin BALB/c mice It may be used as a promising component of sub- unit vaccine.
出处
《世界华人消化杂志》
CAS
2004年第12期2813-2816,共4页
World Chinese Journal of Digestology
基金
贵州省社会发展基金重点课题
No.C-168贵州省优秀人才省长基金课题
No.C-195~~
