摘要
目的 观察肝郁脾虚因素刺激对二乙基亚硝胺诱发大鼠实验性肝癌发生和鸟氨酸脱羧酶表达的影响,以及疏肝健脾方药的调控作用。方法 大鼠造模后选取第 9、14、19 周 3 个时间点分批处死大鼠,取肝组织,用逆转录聚合酶链反应(RT-PCR)法检测鸟氨酸脱羧酶(ODC)表达,以及肝组织病理切片观察。结果 用肝郁脾虚因素刺激二乙基亚硝胺诱发的肝癌大鼠,在第9周后大鼠肝组织表达ODC比单纯用二乙基亚硝胺诱发的肝癌大鼠显著增强,并随着肝癌的发生发展在第 14 周和第 19 周时逐渐增强。结论 肝郁脾虚因素刺激对化学诱发的大鼠实验性肝癌具有显著的促进作用。疏肝健脾中药可以降低肝癌组织细胞中 ODC 的表达,在一定程度上可控制肝癌细胞的增殖。
Objective To observe the influence of restraint stress on the expression of ornithine decarboxylase of chemical hepatocarcinoma in rats. Methods Rats were given diethylnitrosamine 70 mg/kg once a week for 8 weeks. Simultaneously immobilized 8 h/d for 18 weeks. Other rats only given diethylnitrosamine, only immobilized and the normal group were set up as the control. And another group was treated with Chinese medicine Shuganjianpi Decoction. The gene expression of ODC were detected by reverse transcription-polymerase chain reaction (RT-PCR) in each group in the 9th, 14th and 19th week respectively. Results The incidence of hepatocarcinoma in rats given diethylnitrosamine was 100%. Predominant expression of ODC was detected in the hepatocarcinoma group and the immobilization group. The expression intensity of ODC in the group suffered with both hepatocarcinoma and immobilization was significantly higher than that in the control groups, and it became more and more higher in the 14th and 19th week. Conclusion Restraint s t r e s s c o u l d p r o m o t e t h e p r o l i f e r a t i o n a n d m a l i g n r e v e r s i o n o f l i v e r Influence of Restraint Stress on the Gene Eexpression of Ornithine Decarboxylase in Rat with Chemical Hepatocarcinoma GU Li-gang, WANG Ji, ZHANG Qian, et al (Basic Medical College, Beijing University of TCM, Beijing 100029, China) Abstract:Objective To observe the influence of restraint stress on the expression of ornithine decarboxylase of chemical hepatocarcinoma in rats. Methods Rats were given diethylnitrosamine 70 mg/kg once a week for 8 weeks. Simultaneously immobilized 8 h/d for 18 weeks. Other rats only given diethylnitrosamine, only immobilized and the normal group were set up as the control. And another group was treated with Chinese medicine Shuganjianpi Decoction. The gene expression of ODC were detected by reverse transcription-polymerase chain reaction (RT-PCR) in each group in the 9th, 14th and 19th week respectively. Results The incidence of hepatocarcinoma in rats given diethylnitrosamine was 100%. Predominant expression of ODC was detected in the hepatocarcinoma group and the immobilization group. The expression intensity of ODC in the group suffered with both hepatocarcinoma and immobilization was significantly higher than that in the control groups, and it became more and more higher in the 14th and 19th week. Conclusion Restraint stress could promote the proliferation and malign reversion of liver cells. Meanwhile, Shuganjianpi Decoction could inhibit the celluar proliferation.
出处
《中国中医药信息杂志》
CAS
CSCD
2005年第1期35-37,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家中医药管理局科研基金资助项目(37600176)
人事部留学回国人员 A 类重点课题资助项目(2001)
