摘要
目的将体外标记的骨髓基质源神经干细胞经单细胞悬液微移植后观察其在兔纹状体的存活、迁移、分化和整合情况,为细胞移植治疗疾病奠定基础。方法分离兔骨髓基质细胞,利用神经干细胞培养基、白血病抑止因子和碱性成纤维母细胞生长因子进行细胞扩增并诱导成骨髓基质源神经干细胞,再经菲立磁和活细胞荧光染料PKH67标记后,采用微移植的方法,通过脑立体定位仪,用微玻璃针将干细胞分别植入兔脑纹状体内。存活1、4、8周后处死动物,组织切片,利用光镜和电镜观察标记细胞在脑内的形态学情况。结果菲立磁标记的兔骨髓基质源神经干细胞经微移植后可在兔脑内纹状体区域存活,移植的干细胞可向周围的脑实质内迁移和整合,迁移细胞沿特定的纹状体结构分布。少量菲立磁标记的干细胞可以分化成神经元。结论骨髓基质源神经干细胞移植后,可在脑实质内存活、迁移、分化和整合,这种细胞可能成为中枢神经系统自体移植的细胞来源。
Objective To investigate the survival, migration, differentiation and incorporation of Feridex-labeled bone marrow stromal cells derived neural stem cells(BMSCs-D-NSCs) after being transplanted into rabbit striatum with single cell suspension by autologous microtransplantation method and to investigate the possibility of a new cell source for central nervous system transplantation. Methods BMSCs were acquired from the marrow of rabbit thighbone. NSCs culture medium, bFGF and EGF were used to induce BMSCs proliferation and differentiation into BMSCs-D-NSCs in vitro. After these cells were further labeled by Feridex and PKH67, they were transplanted into rabbit striatum by microtransplantation method as single cell suspension with stereoinjection technique. After 1, 4, and 8 weeks, rabbits were sacrificed and their brain tissues were sectioned to investigate the morphology of the transplanted cells under microscopy and electromicroscopy. Results NSCs could survive, migrate and incorporate into the host brain tissue and were distributed along special striatum structure, but a few of them differentiated into neurons. Conclusion The transplanted neural stem cells can survive, migrate, differentiate and incorporate in the rabbit striatum. BMSCs may be a potential source for cell transplantation in treating CNS discorders in humans.
出处
《中华神经医学杂志》
CAS
CSCD
2005年第2期121-125,129,共6页
Chinese Journal of Neuromedicine
基金
国家自然科学基金(30400464
30270491)
中国博士后基金(2003034063)
广东省自然科学基金博士科研启动基金(04300199)
军队(01Z054)
广东省科技厅重大科技项目[粤科基办(2000)25
粤财企(2001)367]
