摘要
目的:阐明风湿性心脏病中钙激活蛋白酶Ⅰ(Calpain Ⅰ)与心房颤动(AF)关系及Calpain Ⅰ在风湿性心脏病不同类型心房颤动时的变化特点。方法:选择心脏手术患者55 例,共分为4 组,A组(n=12):非风湿性心脏病窦性心律组;B组(n=13):风湿性心脏病窦性心律组;C组(n=14):风湿性心脏病阵发性AF组;D组(n=16):风湿性心脏病持续性AF组。在患者右房侧壁组织蛋白提取液不加钙离子或加钙离子(10-3mol/L)的条件下,采用比色法,对非选择性蛋白酶抑制剂E 64、Calpain Ⅰ抑制剂、Calpain Ⅱ抑制剂、溶酶体抑制剂Lactocystin作用于心房肌组织后的蛋白裂解活性进行检测比较。结果:A组、B组在相同试验条件下蛋白裂解活性未见明显差异;不给予蛋白酶抑制剂时, C组、D组的蛋白裂解活性明显高于B组(P<0.01);给予蛋白酶抑制剂,Calpain Ⅰ抑制剂可抑制4组患者心肌组织蛋白裂解活性,而CalpainⅡ抑制剂则不能充分抑制4组患者心肌组织蛋白裂解活性。结论:风心病心房颤动患者心肌组织蛋白裂解活性是升高的,其活性升高与Calpain Ⅰ的作用有密切关系。
Objective:To study the relationship between Calpain Ⅰ and atrial fibrillation in patients with rheumatic heart disease(RHD) and to study Calpain Ⅰ changes in different type of atrial fibrillation. Methods: Fifty patients underwent heart surgery were divided into 4 group. Group A(n=12): Sinus rhythm group without rheumatic heart diseases; Group B(n=13): Sinus rhythm group with RHD; Group C(n=14): Paroxysmal AF with RHD; Group D(n=16): Persistent AF group with RHD.Atrial proteolytic activities in 4 groups were compared with and without Ca 2+ by colorimetric method after atrial myocardial tissue was treated with E-64, Calpain Ⅰ inhibitor,Calpain Ⅱ inhibitor and Lactocystin. Results: No significant differences of proteolytic activities were found between group A and group B under the same condition. Proteolytic activities in group C and group D were significantly higher than those in group B(P<0.01). Calpain Ⅰ inhibitor inhibited atrial proteolytic activities whereas Calpain Ⅱ inhibitor did not. Conclusion: Proteolytic activity increases in atrial fibrillation patients with RHD and the increases has close relationship with Calpain Ⅰ.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2005年第2期134-135,共2页
Academic Journal of Second Military Medical University
基金
国家自然科学基金 (30070749)
