摘要
采用外周血淋巴细胞培养方法和常规染色体制备技术,对内蒙古乌审马染色体核型进行了分析研究。结 果表明:乌审马染色体核型2n=64,雌性核型为64,XX;雄性核型为64,XY。32对染色体中,31对常染色体,1对性 染色体;常染色体中,13对为亚中和中着丝粒染色体,18对为端着丝粒染色体;X染色体为较大的亚中部着丝点染色 体,Y染色体为最小的端着丝点染色体。通过对乌审马的染色体核型分析,为其分子原位杂交及基因定位奠定基础。
In this paper, chromosomal karyotype of Wushen horse in Inner Mongolia was studied by using the peripheral blood lymphocytes culture method and conventional chromosome analysis techniques. The results showed that the diploid chromosome number of Wushen horse was 2n=64. The chromosome karyotypes of male and female horse were 64,XY and 64,XX. Among 31 pairs of autosomes, of which 13 pairs were met centric or submetacentric and 18 pairs acrocentric chromosomes, X chromosome was larger submetacentric chromosome and Y was the smallest acrocentric chromosome. Karyotypes of Wushen horse may lay a foundation of insitu hybridization and location of gene.
出处
《华北农学报》
CSCD
北大核心
2005年第1期108-110,共3页
Acta Agriculturae Boreali-Sinica
基金
内蒙古人才基金项目(B62002)
