摘要
目的建立血浆中伊立替康含量的测定方法.方法以C18色谱柱、0.05mol/LNa2HPO4(pH=3.0,内含0.05mol/L辛烷基磺酸钠)-乙腈=68∶32为流动相分离、荧光检测伊立替康,外标法定量.结果伊立替康浓度在25.0～1000μg/L内,浓度与峰面积之间有良好的线性关系(c=0.00249A-8.15,r=0.9994).最小检出质量浓度为2.0μg/L.50,100,500 μg/L伊立替康的相对回收率(%)分别为96.4、98.3和100.4.三个浓度的平均日内RSD为2.87%,平均日间RSD为4.30%.结论本方法快速、简便、准确,可用于科研和临床工作中伊立替康血药浓度的快速检测.
OBJECTIVE A reversed phase high performance liquid chromatography(RP-HPLC) method was established for determination of irinotecan in human plasma.METHOD Irinotecan was separated on a C_ 18 column at 30℃,monitored at 430(excitation wavelength,370nm). The mobile phase was acetonitrile-0.05mol/L Na_2HPO_4 containing 0.05mol/L sodium octanesulfonate (pH=3.0). RESULTS The calibration curve was linear in the range of 25.0 to 1000μg/L with r=0.9994. The RSD within a day were 1.48%~5.01%(the average value was 2.87%),and the RSD between days were 2.11%~6.26%,the recovery was 96.4%~100.4%. CONCLUSION This method is rapid, simple, accurate and can be used for the determination of plasma irinotecan in clinical and scientific research.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2005年第1期57-59,共3页
Chinese Journal of Modern Applied Pharmacy
