摘要
目的探讨转化生长因子(TGF)β1在梗阻性肾病(UUO)大鼠梗阻肾中致肾小管间质进行性纤维化的作用。方法成年SD大鼠制成UUO模型,在梗阻后3、7、10、14d处死。免疫组织化学染色法对其肾内α平滑肌肌动蛋白(SMA)沉积量及侵入肾内的单核巨噬细胞数进行分析;逆转录多聚酶链反应(RTPCR)对大鼠TGFβ1mRNA、金属蛋白酶组织抑制剂(TIMP)1mRNA及IV型胶原(ColIV)mRNA转录量进行分析。结果随梗阻时间延长,侵入梗阻肾内单核巨噬细胞数、αSMA沉积、ColIVmRNA及TIMP1mRNA含量增加;示肾小管间质存在进行性纤维化。同时TGFβ1mRNA含量明显增加,差异统计学意义(P<0.05)。结论TGFβ1促使梗阻性肾病大鼠肾小管间质进行性纤维化。
Objective To investigate the renal tubule interstitial fibrosis in obstructive nephropathy rats.Methods In adult SD rats,left ureter was ligated to create the model of obstructive nephropathy (UUO).Rats with UUO were sacrificed 3,7,10,and 14 days after obstruction.By using immunohistochemistry,α smooth muscle actin (α-SMA) deposit and the counts of Monocyte/macrophage infiltrating into kidney were analyzed.The expression of transforming growth factor β1 (TGF-β1) mRNA,TIMP-1 mRNA and Col-IV mRNA was detected by using reverse transcription polymerase chain reaction (RT-PCR).Results The deposit of α-SMA,the counts of monocyte/macrophage infiltrating into kidney,the expression of Col-IV mRNA and TIMP-1 mRNA were increased after renal obstruction,suggesting the progressive renal tubule interstitial fibrosis.TGF-β1 was also increased (P<0.05).Conclusion TGF-β1 promoted the renal tubule interstitial fibrosis in rats with UUO.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第3期349-350,共2页
Chinese Journal of Experimental Surgery
