摘要
目的 探讨新生大鼠缺氧缺血性脑损伤 (hypoxic -ischemicbraindamage ,HIBD)后脑组织水通道蛋白 - 4(AQP4 )的表达变化及碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor,bFGF)对其治疗效果的影响。方法 结扎阻断左侧颈总动脉 ,仓内N2 浓度 95 %、O2 浓度 9± 1% ,缺氧持续 2 0min ,建立脑缺氧缺血模型 ,用RT -PCR方法检测出脑缺氧缺血后不同时间段AQP4mRNA基因的表达。并对 7日龄HIBD后新生大鼠进行bFGF治疗 (4μ/ g体重 ,腹腔注射 ,连续 15d)。观察治疗后 5h、3d、7d、15d、17d后AQP4mRNA表达的变化。结果 HIBD后 5h ,脑组织AQP4mRNA表达增加 ;HIBD后 7日达到高峰 (1.93± 0 .11) ,15d后仍高于正常组织 (1.4 1± 0 .2 3) ;HIBD后腹腔注射bFGF ,3d脑组织AQP4mRNA有明显变化 (0 .79± 0 .15 ) ,15d明显降低 (0 .4 7± 0 .0 6 )。结论 AQP4与HIBD的形成密切相关 ;bFGF对HIBD后有明显的改善作用 ,可能与其保护作用有关。
Objective: To investigate the expression of AQP4 in hindbrain of neonatal rats with hypoxic-ischemic brain damage (HIBD), the effects of basic fibroblast growth factor (bFGF) on the treatment of HIBD. Methods: Wistar rats were used to establish HIBD model with the following methods: left common carotid artery was isolated and permanently occluded with a surgical thread, then the rats were exposed to a chamber filled with a mixture of 95% N 2 and 9±1% O 2 for 20 minute. Brain AQP4 mRNA were detected at various intervals with RT-PCR. BFGF were injected into the abdominal cavity of animal models of 15 days old, with the doses of 4(g/kg for 7 days. To observe the expression of AQP4 mRNA in brains at 5 hours, 3 days, 7 days, 15 days, and 15 days injection. Results: AQP4 mRNA increased at 5 hours after HIBD, reached to a peak at 7 days after HIBD (1.93±0.11), remained a high a level at 15 days at 15 days after HIBD (1.41±0.23). After bFGF injection, AQP4 mRNA changed evidently at 3 days (0.79±0.15), decreased obviously at days (0.47±0.06). Conclusions: AQP4 has a lot to do with HIBD; bFGF has anameliorate role for HIBD which may be related to it's protection.
出处
《中国优生与遗传杂志》
2005年第2期24-26,共3页
Chinese Journal of Birth Health & Heredity
