摘要
应用肠杆菌科诊断噬菌体对2280株疑似志贺氏菌进行了检测,同时进行了常规鉴定。结果表明,志贺氏菌属Sh噬菌体10^3RTD对属内裂解率为100%,1RTD为99。9%;65侏与志贺氏菌分型血清呈现凝集的非志贺氏菌,10^3RTD裂解率为12.3%,1RTD为4.6%,裂解模式的测定表明,2215株志贺氏菌分属于7个裂解模式,仅模式3中株鲍氏5型为文献[2,3]所未列入,。余者完全一致。
In this paper , 2280 strains of suspicious Shigella c culture were detected by the diagnostic typing phage set for Enterobacteriaceae, the routine identification of them were carried oat at the same time . The results showed that 100% of shigella cultures were lysed with 10~3RTD and 99.9% lysed with 1RTD of phage Sh. 12.3% of 65 non-shigeUa cultures agglutinating with typing serum of shigeUa was lysed with 10~3RTD, 4.6% lysed with 1RTD of phage Sh. The determination of lytic-pattern of 2215 shigella culture indicated that only 3 strains of Boyd 5 of lytic-pattern 3 were not reported in literature, the rest strains were consistent to fomer studies. The nonshigella cultures lysed by Sh 10~3RTD could be excluded with 1RTD and its lytic-pattern. 20 strains of Enteroinvasive Escherichia coli possessing shigeUa-related antigens could be differentiated by Sh 10~3RTD. 1RTD and its lytic-pattern.
出处
《微生物学通报》
CAS
CSCD
北大核心
1993年第6期339-343,共5页
Microbiology China
关键词
肠杆菌科
志贺氏菌
噬菌体
诊断
Lytic patterns of phage
Enterobacteriaceae
Shigella
Enteroinvasive Escherichia coli