摘要
目的探讨网织血小板(RPs)和P-选择素联合检测在各种血小板减少症中的临床意义。方法采用流式细胞仪技术测定84例血小板减少症眼特发性血小板减少性紫癜穴ITP雪36例,再生障碍性贫血穴AA雪14例,脾功能亢进穴HS雪12例,急性白血病穴AL雪16例,骨髓增生异常综合征穴MDS雪6例演患者全血中RPs百分率和绝对值以及P-选择素水平,观察其在各种血小板减少症中的变化,并与30例正常对照组进行比较。结果(1)正常组RPs百分率(7.72±3.06)%,RPs绝对值(13.34±7.48)×109蛐L,P-选择素水平(2.32±1.78)%;(2)ITP患者RPs百分率高于正常对照组(P<0.01),AA、HS、AL、MDS患者RPs百分率与正常对照组比较差别无显著性意义(P>0.05),ITP、AA、HS、AL、MDS患者RPs绝对值均低于正常对照组(P<0.05或0.01);(3)ITP、AL、MDS患者P-选择素水平明显高于正常对照组(P<0.05或0.01),AA、HS患者P-选择素与正常对照组相似(P>0.05)。结论RPs百分率、RPs绝对值和P-选择素水平能反映血小板减少症患者骨髓血小板的生成能力及体内血小板的活化破坏状况,两者联合检测有助于对血小板减少症的鉴别。
Objective To investigate the clinical significance of reticulat ed platelets (RPs) and P-selectin levels in thrombocytopenic diseases. Methods The percentage and absolute counts of RPs and P-selectin levels were detected in 30 normal subjects and 84 patients with various thrombocytopenic diseases, incl uding 36 cases of idiopathic thrombocytopenic purpura (ITP), 14 cases of aplasti c anemia (AA), 12 cases of hypersplenism, 16 cases of acute leukemia (AL) and 6 cases of myelodysplasia syndrome (MDS) by flow cytometry (FCM). Results (1) In the normal controls, RPs% was(7.72±3.06)%, the RPs absolute counts were(13.34± 7.48)×109/L, and the P-selectin level was (2.32±1.78)%. (2) As compared with the normal controls, RPs% in patients with ITP was significantly higher(P<0.01), while in the patients with AA,HS, AL and MDS, there was no significant differe nce(P>0.05). RPs absolute counts in the patients with ITP was lower than that in the normal controls(P<0.05), and that in patients with AA, HS, AL, MDS were als o lower than that in normal controls(P<0.01); (3) The P-selectin level in patien ts with ITP, AL and MDS was significantly higher than that of normal controls(P< 0.01), 0.001 and 0.05 respectively, and there were no significant differences fo r patients with AA and hypersplenism(P>0.05). Conclusion The measurement of RPs and P-selectin can be used to differentiate various conditions with thrombocyto penia.
出处
《浙江医学》
CAS
2005年第2期92-94,共3页
Zhejiang Medical Journal
基金
浙江省教育厅科研基金(20020443)
