摘要
目的 探讨HBV -M阳性患者血清中HBV -DNA含量与血清中TBiL、DBiLABiL、AST、ALT、AST/ALT、ALP、γ -GT、TBA等 9项指标间的相关性。方法 将 10 6份HBV -M阳性标本分为 3组 :Ⅰ组HBV -DNA含量 <10 0 0拷贝 /毫升的小三阳者 ;Ⅱ组为HBV -DNA含量 10 5- 10 6拷贝 /毫升血清的小三阳者 ;Ⅲ组为HBV -DNA含量为 10 7- 10 9拷贝 /毫升血清的大三阳者。HBV -M采用ELISA法 ,HBV -DNA采用美国PE公司生产的PE - 5 70 0型FQ -PCR检测仪测定 ;肝功能 9项指标采用美国贝克曼公司CE -CX5全自动生化分析仪检测。HBV -DNA与肝功能指标测定使用同一份标本。结果 经每组比较分析后 ,大部分肝功能指标均有显著性或非常显著性差别 ,其中以Ⅱ组各项指标差异尤为显著。特别是ALT、AST、TBA等。结论 通过相关性分析HBV -DNA含量与肝功能 9项指标之间没有明显的相关性 (r=± 0 .2以内 P >0 .0 5 ) ,肝功能的损害程度与HBV -DNA含量之间无相关性。定量检测外周血HBV -DNA可以直接反应HBV在血液中的复制情况 ,既而能够较为准确地解释感染HBV后的传染性。
Objective To study the relativity among the quantity of HBV-DNA inthe blood serum to the nine indexes (TBiL, DBiL, AST,ALT,AST/ALT, ALP,γ-GT,TBA) of the ability of the liver ,the HBV-M of the patients are active.Methods 106 samples (the HBV-M are active )are divided into three groups , the first group is that the quantity of HBV-DNA shorten 1000 copy/ml and the HbsAg,CORE,Anti-Hbe are active ; the second group is that the quantity of HBV-DNA is 10 5-10 6 copy/ml and the HbsAg , CORE, Anti-Hbe are active; the third group is that the quantity of HBV-DNA is 10 7-10 9 copy/ml and the HbsAg,CORE,Hbe are active; Measuring the quantity of HBV-M uses the method of ELISA, the quantity of HBV-DNA uses the method of FQ-PCR(PE-5700 analyzer)(PE Corp. American),the measuring of the nine indexes of the liver ability uses the automatic biochemical analyzer CE-CX5 (the Beckman Corp. American ).The both use the same sample.Results After being analyzed, the most indexes of the liver ability have significant difference or much significant difference, the difference of the indexes of the liver ability in the second group are more significant, especially the indexes of ALT,AST,TBA are notable.Conclusion Analyzing the relativity, the quantity of HBV-DNA in the blood serum haven't significant relativity to the nine indexes of the liver ability (r=±0.2,P>0.05), the injury degree of the ability of liver did not related to the quantity of HBV-DNA. Measuring the quantity of HBV-DNA of peripheral blood may reflect directly the reproduce of HBV virus in the blood, also could exactly interpret the infection of HBV after being contracted.
出处
《宁夏医学杂志》
CAS
2005年第2期84-86,共3页
Ningxia Medical Journal
