摘要
背景:以往单方面研究低氧或低温对机体的影响比较多,目前低氧和低温两个因素综合作用的研究逐渐引起医学界的重视。目的:探讨急性低温低氧这两因素同时作用对正常家兔和心肌缺血家兔超氧化物歧化酶(superoxidedismutase,SOD)、丙二醛的影响。设计:随机对照试验。地点、材料和干预:实验地点为右江民族医学院应用生理研究室。实验选用健康家兔32只,体质量1.8～2.5kg。采用随机数字法将家兔分成4组,每组均8只:常温常氧组、低温低氧组、心肌缺血常温常氧组、心肌缺血低温低氧组。将正常家兔和心肌缺血家兔置于低温犤(-10±2)℃犦和低氧(氧含量为8.5%)冷柜中1h,然后测定血清中SOD活力和丙二醛含量。主要观察指标:正常和心肌缺血家兔在急性低氧条件下血清SOD和丙二醛水平比较。结果:低温低氧组家兔血清中的SOD犤(371.04±29.96)kNU/L犦明显低于常温常氧组犤(424.09±22.59)kNU/L犦(t=5.21,P<0.01),丙二醛犤(5.58±0.44)μmol/L〗高于常温常氧组犤(4.44±1.11)μmol/L犦(t=3.21,P<0.05)。心肌缺血低温低氧组家兔血清中的SOD明显低于心肌缺血常温常氧组(t=4.37,P<0.01),丙二醛明显高于心肌缺血常温常氧组(t=2.94,P<0.05)。结论:急性低温低氧共同作用可加重机体的损伤。
BACKGROUND:Previously,there are some researches on studying the effects of hypoxia or hypothermia singly.Nowadays,study on the synthetic action of hypoxia and hypothermia has been attached widely importance. OBJECTIVE:To explore the combination effects of acute hypothermia and hypoxia on superoxide dismutase(SOD) and malondialdehyde in normal rabbits and rabbits with myocardial ischemia. DESIGN:Randomized controlled trial. SETTING,MATERIALS and INTERVENTIONS:Experimental site:Physiological Laboratory of Youjiang Ethnic Medical College.A total of 32 healthy rabbits weighed between 1.8 to 2.5 kg were chosen.They were randomly divided into 4 groups each with 8 rabbits:normal group,hypothermia and hypoxia group,ischemic group with normal temperature and oxygen,ischemic group with hypothermia and hypoxia.Normal rabbits and ischemic rabbits were put in fridge at low temperature(-10±2) ℃and low oxygen(oxygen content of 8.5%) for one hour and tested activity of SOD and concentration of malondialdehyde in serum. MAIN OUTCOME MEASURES:Comparison of serum SOD and malondialdehyde between normal rabbits and myocardial ischemic rabbits under the acute hypothermia and hypoxia conditions. RESULTS:The SOD level of rabbits in hypothermia and hypoxia group[(371.04±29.96) kNU/L] was much lower than that of normal temperature and oxygen group[(424.09±22.59) kNU/L](t=5.21,P< 0.01) while the content of malondialdehyde[(5.58±0.44) μmol/L] was greater than that of normal group[(4.44±1.11) μmol/L](t=3.21,P< 0.05).In myocardial ischemia group with hypothermia and hypoxia,SOD was greatly lower than that of myocardial ischemia group with normal temperature and oxygen(t=4.37,P< 0.01) while the malondialdehyde was greatly higher than that of ischemic group with normal temperature and oxygen group(t=2.94,P< 0.05). CONCLUSION:The combined effects of acute hypothermia and hypoxia will aggravate the damage to body.
出处
《中国临床康复》
CSCD
北大核心
2005年第7期184-185,共2页
Chinese Journal of Clinical Rehabilitation
基金
广西科技厅资助课题(2001041)~~
