摘要
目的:探讨核转录因子-κB家族成员中的一个重要亚基p65及其上游激酶IKKα在金地鼠颊囊鳞癌发生 过程中的表达及意义。方法:建立金地鼠颊囊癌变的动物模型。采用Westernblot法检测12组配对金地鼠正常颊 黏膜、上皮单纯增生、上皮异常增生和鳞癌组织所提取的核蛋白中p65的表达差异。采用SABC免疫组织化学方 法,检测各组IKKα的表达变化。结果:在正常颊黏膜和上皮单纯增生组织中,未见明显的p65蛋白质印迹带,且两 者相比无显著性差异(P>0.05);在正常颊囊黏膜中IKKα的表达几乎呈阴性,黏膜上皮的各细胞层细胞未见明显 的胞浆着色。上皮单纯增生组织染色类似正常上皮。随着上皮异常增生的出现,p65蛋白质印迹带增强,较正常组 和单纯增生组均有显著性差异(P<0.01);同时,与正常组和单纯增生组相比,IKKα的阳性着色强度增强,阳性细 胞数增加,并与异常增生程度有关(P<0.01)。在鳞癌组织中,p65蛋白质印迹带明显增强,与正常组和异常增生 组相比均有显著性差异(P<0.01);而IKKα的阳性表达进一步增加,呈广泛的强阳性表达。与正常组和异常增生 组相比差异均有显著性(P<0.01)。结论:p65在金地鼠颊囊鳞癌发生、发展过程中被激活。p65和IKKα在金地 鼠颊囊癌变过程中表达异常。
Objective: To study the expression and significance of p65, an important subtype of nuclear transcription factor κB (NF-κB) and its upstream kinase IKKα in malignant transformation of hamster buccal mucosa. Methods: We established the animal model of malignant transformation in hamster buccal mucosa induced by DMBA(0.5%). Twelve paired specimens including normal buccal mucosa, epithelial hyperplasia, epithelial dysplasia and squamous cell carcinoma (SCC) were subjected to Western blot for analysis p65.The expression of IKKα was observed in the four different groups by immunohistochemical evaluation. Results: In normal buccal epithelia and hyperplasia epithelia, evident Western blotting band of p65 was not found, and there was no significant difference between them(P>0.05);the expression of IKKα in normal buccal pouch mucous membrane was almost negative, and evident plasm staining was not found in the cellular layers of mucous membrane epithelia. The staining of hyperplasia epithelia was similar to that of normal buccal epithelia. With the occurrence of epithelia dysplasia ,the expression of p65 gradually increased, and significant difference could be found when comparing with normal buccal epithelia and hyperplasia epithelia(P<0.01); at the same time, comparing with normal buccal mucosa and epithelial hyperplasia, the positive staining intensity of IKKα raised with the increase of positive cells, which had some relations with the degree of epithelia dysplasia. In SCC, p65 was expressed to a higher level comparing with normal buccal mucosa and dysplasia(P<0.01), while the positive expression of IKKα increased significantly with the broad positive expression. Significant differences could be found when comparing with normal buccal epithelia and epithelial dysplasia (P<0.01). Conclusions: NF-κB p65 is strongly activated in malignant transformation of hamster buccal mucosa. P65 and IKKα expressed abnormally in malignant transformation of hamster buccal mucosa.
出处
《口腔医学研究》
CAS
CSCD
2005年第1期31-34,共4页
Journal of Oral Science Research
